Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.1.B26 extracted from

  • Thompson, P.R.; Hughes, D.W.; Wright, G.R.
    Mechanism of aminoglycoside 3'-phosphotransferase type IIIa: His188 is not a phosphate-accepting residue (1996), Chem. Biol., 3, 747-755.
    View publication on PubMed
Search for more literature for 2.7.1.B26

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Enterococcus faecium

Protein Variants

Protein Variants Comment Organism
H123A mutation has minimal effects on phosphorylation of several aminoglycosides Enterococcus faecium
H188A mutation has minimal effects on phosphorylation of several aminoglycosides Enterococcus faecium
H78A mutation has minimal effects on phosphorylation of several aminoglycosides Enterococcus faecium
H82A mutation has minimal effects on phosphorylation of several aminoglycosides Enterococcus faecium

Inhibitors

Inhibitors Comment Organism Structure
1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide 10 mM, 2% residual activity Enterococcus faecium
4-chloromercuribenzoic acid 0.5 mM, 3% residual activity Enterococcus faecium
diethyldicarbonate 2.5 mM, 10% residual activity Enterococcus faecium
kanamycin A
-
 Enterococcus faecium
N-ethylmaleimide 0.5 mM, 47% residual activity Enterococcus faecium
neomycin B
-
 Enterococcus faecium
Phenylglyoxal 2.5 mM, 10% residual activity Enterococcus faecium
Tetranitromethane 2.5 mM, 23% residual activity Enterococcus faecium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00029
-
 neomycin B mutant H123A, pH 7.5, 37°C Enterococcus faecium
0.00092
-
 kanamycin A mutant H123A, pH 7.5, 37°C Enterococcus faecium
0.00116
-
 ATP mutant H123A, pH 7.5, 37°C Enterococcus faecium
0.0013
-
 neomycin B mutant H82A, pH 7.5, 37°C Enterococcus faecium
0.0024
-
 kanamycin A mutant H82A, pH 7.5, 37°C Enterococcus faecium
0.0032
-
 neomycin B mutant H188A, pH 7.5, 37°C Enterococcus faecium
0.0051
-
 kanamycin A mutant H78A, pH 7.5, 37°C Enterococcus faecium
0.00772
-
 neomycin B wild-type, pH 7.5, 37°C Enterococcus faecium
0.011
-
 ATP mutant H78A, pH 7.5, 37°C Enterococcus faecium
0.012
-
 ATP mutant H82A, pH 7.5, 37°C Enterococcus faecium
0.0126
-
 kanamycin A wild-type, pH 7.5, 37°C Enterococcus faecium
0.0174
-
 kanamycin A mutant H188A, pH 7.5, 37°C Enterococcus faecium
0.027
-
 neomycin B mutant H78A, pH 7.5, 37°C Enterococcus faecium
0.0277
-
 ATP wild-type, pH 7.5, 37°C Enterococcus faecium
0.0335
-
 ATP mutant H188A, pH 7.5, 37°C Enterococcus faecium
0.093
-
 amikacin mutant H82A, pH 7.5, 37°C Enterococcus faecium
0.11
-
 amikacin mutant H123A, pH 7.5, 37°C Enterococcus faecium
0.184
-
 amikacin mutant H188A, pH 7.5, 37°C Enterococcus faecium
0.206
-
 amikacin mutant H78A, pH 7.5, 37°C Enterococcus faecium
0.245
-
 amikacin wild-type, pH 7.5, 37°C Enterococcus faecium

Organism

Organism UniProt Comment Textmining
Enterococcus faecium E9LMR8
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + amikacin
-
 Enterococcus faecium ADP + amikacin 3'-phosphate
-
 ?
ATP + kanamycin A
-
 Enterococcus faecium ADP + kanamycin A 3'-phosphate
-
 ?
ATP + neomycin B
-
 Enterococcus faecium ADP + neomycin B 3'-phosphate
-
 ?

Synonyms

Synonyms Comment Organism
APH-3'-IIIa
-
 Enterococcus faecium
Aph3
-
 Enterococcus faecium

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.35
-
 ATP mutant H82A, pH 7.5, 37°C Enterococcus faecium
0.35
-
 kanamycin A mutant H82A, pH 7.5, 37°C Enterococcus faecium
0.49
-
 kanamycin A mutant H123A, pH 7.5, 37°C Enterococcus faecium
0.5
-
 amikacin mutant H82A, pH 7.5, 37°C Enterococcus faecium
0.57
-
 ATP mutant H123A, pH 7.5, 37°C Enterococcus faecium
0.66
-
 ATP mutant H78A, pH 7.5, 37°C Enterococcus faecium
0.67
-
 kanamycin A mutant H78A, pH 7.5, 37°C Enterococcus faecium
0.69
-
 neomycin B mutant H82A, pH 7.5, 37°C Enterococcus faecium
0.83
-
 neomycin B mutant H123A, pH 7.5, 37°C Enterococcus faecium
0.87
-
 amikacin mutant H123A, pH 7.5, 37°C Enterococcus faecium
1.2
-
 neomycin B mutant H188A, pH 7.5, 37°C Enterococcus faecium
1.74
-
 amikacin mutant H78A, pH 7.5, 37°C Enterococcus faecium
1.76
-
 ATP wild-type, pH 7.5, 37°C Enterococcus faecium
1.79
-
 kanamycin A wild-type, pH 7.5, 37°C Enterococcus faecium
2.04
-
 amikacin mutant H188A, pH 7.5, 37°C Enterococcus faecium
2.08
-
 neomycin B wild-type, pH 7.5, 37°C Enterococcus faecium
2.46
-
 amikacin wild-type, pH 7.5, 37°C Enterococcus faecium
2.46
-
 neomycin B mutant H78A, pH 7.5, 37°C Enterococcus faecium
3.36
-
 kanamycin A mutant H188A, pH 7.5, 37°C Enterococcus faecium
3.75
-
 ATP mutant H188A, pH 7.5, 37°C Enterococcus faecium

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.68
-
 kanamycin A mutant H78A, pH 7.5, 37°C Enterococcus faecium
1.84
-
 neomycin B mutant H78A, pH 7.5, 37°C Enterococcus faecium
2.65
-
 neomycin B wild-type, pH 7.5, 37°C Enterococcus faecium
2.74
-
 neomycin B mutant H188A, pH 7.5, 37°C Enterococcus faecium
5.03
-
 kanamycin A mutant H123A, pH 7.5, 37°C Enterococcus faecium
6.38
-
 kanamycin A wild-type, pH 7.5, 37°C Enterococcus faecium
6.7
-
 kanamycin A mutant H82A, pH 7.5, 37°C Enterococcus faecium
6.95
-
 neomycin B mutant H123A, pH 7.5, 37°C Enterococcus faecium