Inhibitors | Comment | Organism | Structure |
---|---|---|---|
kanamycin | - |
Enterococcus faecium | |
neamine | - |
Enterococcus faecium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0071 | - |
3-deaminoneamine | pH not specified in the publication, temperature not specified in the publication | Enterococcus faecium | |
0.0126 | - |
kanamycin A | pH not specified in the publication, temperature not specified in the publication | Enterococcus faecium | |
0.02 | - |
neamine | pH not specified in the publication, temperature not specified in the publication | Enterococcus faecium | |
0.0359 | - |
2'-deaminoneamine | pH not specified in the publication, temperature not specified in the publication | Enterococcus faecium | |
0.0518 | - |
1-deaminoneamine | pH not specified in the publication, temperature not specified in the publication | Enterococcus faecium | |
0.0818 | - |
6'-deaminoneamine | pH not specified in the publication, temperature not specified in the publication | Enterococcus faecium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterococcus faecium | E9LMR8 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | protein contains no disulfide bonds | Enterococcus faecium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + 1-deaminoneamine | - |
Enterococcus faecium | GDP + 1-deaminoneamine 3'-phosphate | - |
? | |
GTP + 2'-deaminoneamine | - |
Enterococcus faecium | GDP + 2'-deaminoneamine 3'-phosphate | - |
? | |
GTP + 3-deaminoneamine | - |
Enterococcus faecium | GDP + 3-deaminoneamine 3'-phosphate | - |
? | |
GTP + 6'-deaminoneamine | - |
Enterococcus faecium | GDP + 6'-deaminoneamine 3'-phosphate | - |
? | |
GTP + kanamycin A | - |
Enterococcus faecium | GDP + kanamycin A 3'-phosphate | - |
? | |
GTP + neamine | - |
Enterococcus faecium | GDP + neamine 3'-phosphate | - |
? | |
additional information | an amino group on C-1 and either an amino or a hydroxyl group at the 2' and 6' positions are important for detoxification of aminoglycosides by the enzyme | Enterococcus faecium | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
APH-3'-IIIa | - |
Enterococcus faecium |
Aph3 | - |
Enterococcus faecium |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.278 | - |
6'-deaminoneamine | pH not specified in the publication, temperature not specified in the publication | Enterococcus faecium | |
0.39 | - |
2'-deaminoneamine | pH not specified in the publication, temperature not specified in the publication | Enterococcus faecium | |
1.03 | - |
1-deaminoneamine | pH not specified in the publication, temperature not specified in the publication | Enterococcus faecium | |
1.16 | - |
3-deaminoneamine | pH not specified in the publication, temperature not specified in the publication | Enterococcus faecium | |
1.76 | - |
kanamycin A | pH not specified in the publication, temperature not specified in the publication | Enterococcus faecium | |
2 | - |
neamine | pH not specified in the publication, temperature not specified in the publication | Enterococcus faecium |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | - |
neamine | pH not specified in the publication, temperature not specified in the publication | Enterococcus faecium | |
6.3 | - |
kanamycin | pH not specified in the publication, temperature not specified in the publication | Enterococcus faecium |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.4 | - |
kanamycin A | pH not specified in the publication, temperature not specified in the publication | Enterococcus faecium | |
11 | - |
6'-deaminoneamine | pH not specified in the publication, temperature not specified in the publication | Enterococcus faecium | |
20 | - |
3-deaminoneamine | pH not specified in the publication, temperature not specified in the publication | Enterococcus faecium | |
100 | - |
1-deaminoneamine | pH not specified in the publication, temperature not specified in the publication | Enterococcus faecium | |
160 | - |
2'-deaminoneamine | pH not specified in the publication, temperature not specified in the publication | Enterococcus faecium |