Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.1.B26 extracted from

  • McKay, G.A.; Roestamadji, J.; Mobashery, S.; Wright, G.D.
    Recognition of aminoglycoside antibiotics by enterococcal-staphylococcal aminoglycoside 3'-phosphotransferase type IIIa: role of substrate amino groups (1996), Antimicrob. Agents Chemother., 40, 2648-2650.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
kanamycin
-
Enterococcus faecium
neamine
-
Enterococcus faecium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0071
-
3-deaminoneamine pH not specified in the publication, temperature not specified in the publication Enterococcus faecium
0.0126
-
kanamycin A pH not specified in the publication, temperature not specified in the publication Enterococcus faecium
0.02
-
neamine pH not specified in the publication, temperature not specified in the publication Enterococcus faecium
0.0359
-
2'-deaminoneamine pH not specified in the publication, temperature not specified in the publication Enterococcus faecium
0.0518
-
1-deaminoneamine pH not specified in the publication, temperature not specified in the publication Enterococcus faecium
0.0818
-
6'-deaminoneamine pH not specified in the publication, temperature not specified in the publication Enterococcus faecium

Organism

Organism UniProt Comment Textmining
Enterococcus faecium E9LMR8
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information protein contains no disulfide bonds Enterococcus faecium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + 1-deaminoneamine
-
Enterococcus faecium GDP + 1-deaminoneamine 3'-phosphate
-
?
GTP + 2'-deaminoneamine
-
Enterococcus faecium GDP + 2'-deaminoneamine 3'-phosphate
-
?
GTP + 3-deaminoneamine
-
Enterococcus faecium GDP + 3-deaminoneamine 3'-phosphate
-
?
GTP + 6'-deaminoneamine
-
Enterococcus faecium GDP + 6'-deaminoneamine 3'-phosphate
-
?
GTP + kanamycin A
-
Enterococcus faecium GDP + kanamycin A 3'-phosphate
-
?
GTP + neamine
-
Enterococcus faecium GDP + neamine 3'-phosphate
-
?
additional information an amino group on C-1 and either an amino or a hydroxyl group at the 2' and 6' positions are important for detoxification of aminoglycosides by the enzyme Enterococcus faecium ?
-
?

Synonyms

Synonyms Comment Organism
APH-3'-IIIa
-
Enterococcus faecium
Aph3
-
Enterococcus faecium

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.278
-
6'-deaminoneamine pH not specified in the publication, temperature not specified in the publication Enterococcus faecium
0.39
-
2'-deaminoneamine pH not specified in the publication, temperature not specified in the publication Enterococcus faecium
1.03
-
1-deaminoneamine pH not specified in the publication, temperature not specified in the publication Enterococcus faecium
1.16
-
3-deaminoneamine pH not specified in the publication, temperature not specified in the publication Enterococcus faecium
1.76
-
kanamycin A pH not specified in the publication, temperature not specified in the publication Enterococcus faecium
2
-
neamine pH not specified in the publication, temperature not specified in the publication Enterococcus faecium

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2
-
neamine pH not specified in the publication, temperature not specified in the publication Enterococcus faecium
6.3
-
kanamycin pH not specified in the publication, temperature not specified in the publication Enterococcus faecium

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4
-
kanamycin A pH not specified in the publication, temperature not specified in the publication Enterococcus faecium
11
-
6'-deaminoneamine pH not specified in the publication, temperature not specified in the publication Enterococcus faecium
20
-
3-deaminoneamine pH not specified in the publication, temperature not specified in the publication Enterococcus faecium
100
-
1-deaminoneamine pH not specified in the publication, temperature not specified in the publication Enterococcus faecium
160
-
2'-deaminoneamine pH not specified in the publication, temperature not specified in the publication Enterococcus faecium