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Literature summary for 2.7.1.95 extracted from

  • Elizarov, S.M.; Alekseeva, M.G.; Novikov, F.N.; Chilov, G.G.; Maslov, D.A.; Shtil, A.A.; Danilenko, V.N.
    Identification of phosphorylation sites in aminoglycoside phosphotransferase VIII from Streptomyces rimosus (2012), Biochemistry (Moscow), 77, 1258-1265.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
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Streptomyces rimosus

Protein Variants

Protein Variants Comment Organism
S146A 19% of the activity of phosphorylated wild-type, 90% of the activity of unphosphorylated wild-type, in presence of Ca2+ Streptomyces rimosus
S146A/S160A 17% of the activity of phosphorylated wild-type, 85% of the activity of unphosphorylated wild-type, in presence of Ca2+ Streptomyces rimosus
S146A/S160A/S215A 17% of the activity of phosphorylated wild-type, 60% of the activity of unphosphorylated wild-type, in presence of Ca2+ Streptomyces rimosus
S215A 88% of the activity of phosphorylated wild-type, 67% of the activity of unphosphorylated wild-type, in presence of Ca2+ Streptomyces rimosus
S95A/S160A 82% of the activity of phosphorylated wild-type, 95% of the activity of unphosphorylated wild-type, in presence of Ca2+ Streptomyces rimosus

Organism

Organism UniProt Comment Textmining
Streptomyces rimosus
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Streptomyces rimosus ATCC 10970
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Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein residue Ser146 in the activation loop of the enzyme is the key site for Ca2+-dependent phosphorylation of isoform APHVIII. The Ser146 modification leads to a 6-7-fold increase in the kanamycin kinase activity of APHVII Streptomyces rimosus

Synonyms

Synonyms Comment Organism
aminoglycoside phosphotransferase VIII
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Streptomyces rimosus
APH
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Streptomyces rimosus