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Literature summary for 2.7.1.94 extracted from
- A novel acylglycerol kinase that produces lysophosphatidic acid modulates cross talk with EGFR in prostate cancer cells (2005), J. Cell. Biol., 169, 801-811.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis | Mus musculus |
| DNA and amino acid sequence determination and analysis, expression in human PC-3 cells and HEK-293 cells, in mouse NIH 3T3 cells, and in rat RH7777 cells | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| G126E | site-directed mutagenesis, mutation of the conserved glycine residue of the glycine-rich loop of the ATP binding site, catalytically inactive mutant | Homo sapiens |
| additional information | inactivation of all genes by siRNA transfection, overview | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| octyl-beta-glucopyranoside | inhibits the recombinant enzyme in cell extract of transfected NIH 3T3 cells | Homo sapiens |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Mus musculus | 5739 | - |
| mitochondrion | - |
Homo sapiens | 5739 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + diacylglycerol | Homo sapiens | - |
ADP + phosphatidic acid | i.e. diacyl-glycerol 3-phosphate, regulation mechanism, overview | ? | |
| ATP + monoacylglycerol | Homo sapiens | - |
ADP + lysophosphatidic acid | i.e. acyl-sn-glycerol 3-phosphate, regulation mechanism, overview | ? | |
| additional information | Homo sapiens | lysophosphatidic acid induces the cytokine interleukin-8 correlated with angiogenesis, tumorigenicity, and cancer cell proliferation, the enzyme modulates cross talk with EGFR in prostate cancer cells, enzyme regulates the synthesis of the bioactive phospholipids lysophosphatidic acid and phosphatidic acid, activation of extracellular signal related kinase 1 and 2, thus being involved in pathenogenesis of cancer via enhanced cell proliferation | ? | - |
? | |
| additional information | Mus musculus | the enzyme modulates cross talk with EGFR in prostate cancer cells, enzyme regulates the synthesis of the bioactive phospholipids lysophosphatidic acid and phosphatidic acid, activation of extracellular signal related kinase 1 and 2, thus being involved in pathenogenesis of cancer via enhanced cell proliferation | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Mus musculus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cervical carcinoma cell | high expression level | Homo sapiens | - |
| colon | low expression in normal and very low expression level in tumor cells | Homo sapiens | - |
| gastric cancer cell | high expression level | Homo sapiens | - |
| heart | - |
Homo sapiens | - |
| kidney | - |
Homo sapiens | - |
| kidney tumor cell | - |
Homo sapiens | - |
| additional information | no expression in ovary, lung, small intestine, and thymus | Homo sapiens | - |
| PC-3 cell | prostate cancer cell line | Homo sapiens | - |
| prostate | - |
Homo sapiens | - |
| prostate cancer cell | enzyme expression is upregulated in prostate cancer | Homo sapiens | - |
| rectum | low expression level in normal and tumor cells | Homo sapiens | - |
| uterine cancer cell | high expression level | Homo sapiens | - |
| uterine cervix | low expression level | Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 1,2-dioleoyl-sn-glycerol | recombinant enzyme | Homo sapiens | ADP + 1,2-dioleoyl-sn-glycerol 3-phosphate | - |
? | |
| ATP + 1-oleoyl-sn-glycerol | recombinant enzyme | Homo sapiens | ADP + 1-oleoyl-sn-glycerol 3-phosphate | - |
? | |
| ATP + 1-palmitoyl-sn-glycerol | recombinant enzyme | Homo sapiens | ADP + 1-palmitoyl-sn-glycerol 3-phosphate | - |
? | |
| ATP + 1-stearoyl-sn-glycerol | recombinant enzyme | Homo sapiens | ADP + 1-stearoyl-sn-glycerol 3-phosphate | - |
? | |
| ATP + 2-arachidonoyl-glycerol | recombinant enzyme | Homo sapiens | ADP + 2-arachidonoyl-glycerol 3-phosphate | - |
? | |
| ATP + diacylglycerol | - |
Homo sapiens | ADP + phosphatidic acid | i.e. diacyl-glycerol 3-phosphate, regulation mechanism, overview | ? | |
| ATP + diacylglycerol | - |
Mus musculus | ADP + phosphatidic acid | i.e. diacyl-glycerol 3-phosphate | ? | |
| ATP + diacylglycerol | - |
Homo sapiens | ADP + phosphatidic acid | i.e. diacyl-glycerol 3-phosphate | ? | |
| ATP + monoacylglycerol | - |
Homo sapiens | ADP + lysophosphatidic acid | i.e. acyl-sn-glycerol 3-phosphate, regulation mechanism, overview | ? | |
| ATP + monoacylglycerol | - |
Mus musculus | ADP + lysophosphatidic acid | i.e. acyl-sn-glycerol 3-phosphate | ? | |
| ATP + monoacylglycerol | - |
Homo sapiens | ADP + lysophosphatidic acid | i.e. acyl-sn-glycerol 3-phosphate | ? | |
| additional information | lysophosphatidic acid induces the cytokine interleukin-8 correlated with angiogenesis, tumorigenicity, and cancer cell proliferation, the enzyme modulates cross talk with EGFR in prostate cancer cells, enzyme regulates the synthesis of the bioactive phospholipids lysophosphatidic acid and phosphatidic acid, activation of extracellular signal related kinase 1 and 2, thus being involved in pathenogenesis of cancer via enhanced cell proliferation | Homo sapiens | ? | - |
? | |
| additional information | the enzyme modulates cross talk with EGFR in prostate cancer cells, enzyme regulates the synthesis of the bioactive phospholipids lysophosphatidic acid and phosphatidic acid, activation of extracellular signal related kinase 1 and 2, thus being involved in pathenogenesis of cancer via enhanced cell proliferation | Mus musculus | ? | - |
? | |
| additional information | no activity by the recombinant enzyme with C6-ceramide and sphingosine | Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AGK | - |
Mus musculus |
| AGK | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
assay at | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Mus musculus | |
| ATP | - |
Homo sapiens | |
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Release 2023.1 (January 2023)
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