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Literature summary for 2.7.1.91 extracted from
- Phosphorylation of the immunomodulatory drug FTY720 by sphingosine kinases (2003), J. Biol. Chem., 278, 47408-47415.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| isozymes SPHK1 and SPHK2, expression in HEK293 cells as EGFP-fusion proteins | Mus musculus |
| isozymes SPHK1 and SPHK2, several splicing variants are differently active, expression in HEK293 cells as EGFP-fusion proteins | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics, isozymes SPHK1 and SPHK2 | Homo sapiens | |
| additional information | - |
additional information | kinetics, isozymes SPHK1 and SPHK2 | Mus musculus | |
| 0.013 | - |
sphingosine | pH 7.4, 30°C, recombinant isozyme SPHK1a | Mus musculus |  |
| 0.013 | - |
sphingosine | pH 7.4, 30°C, recombinant isozyme SPHK2 | Mus musculus | |
| 0.014 | - |
sphingosine | pH 7.4, 30°C, recombinant isozyme SPHK2 | Homo sapiens | |
| 0.016 | - |
sphingosine | pH 7.4, 30°C, recombinant isozyme SPHK1 | Homo sapiens | |
| 0.018 | - |
FTY720 | pH 7.4, 30°C, recombinant isozyme SPHK2 | Homo sapiens | |
| 0.024 | - |
FTY720 | pH 7.4, 30°C, recombinant isozyme SPHK2 | Mus musculus | |
| 0.692 | - |
FTY720 | about, pH 7.4, 30°C, recombinant isozyme SPHK1a | Mus musculus | |
| 0.785 | - |
FTY720 | about, pH 7.4, 30°C, recombinant isozyme SPHK1 | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Homo sapiens | 5829 | - |
| cytosol | major part of isozyme SPHK1 and isozyme SPHK2 | Mus musculus | 5829 | - |
| particle-bound | - |
Mus musculus | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + sphinganine | Homo sapiens | - |
ADP + sphinganine 1-phosphate | - |
? | |
| ATP + sphinganine | Mus musculus | - |
ADP + sphinganine 1-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q9NYA1 | isozyme SPHK1; 2 isozymes SPHK1 and SPHK2, several splicing variants | - |
| Homo sapiens | Q9NRA0 | isozyme SPHK2; 2 isozymes SPHK1 and SPHK2, several splicing variants | - |
| Mus musculus | Q9JIA7 | isozyme SPHK2; 2 isozymes SPHK1 and SPHK2, several splicing variants | - |
| Mus musculus | O88885 | isozyme SPHK1; 2 isozymes SPHK1 and SPHK2, several splicing variants | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| blood | higher activity of isozyme SPHK1, low activity of isozyme SPHK2 | Homo sapiens | - |
| blood | higher activity of isozyme SPHK1, low activity of isozyme SPHK2 | Mus musculus | - |
| brain | quite low activity of isozyme SPHK1 and isozyme SPHK2 | Mus musculus | - |
| endothelial cell | - |
Homo sapiens | - |
| kidney | isozyme SPHK1 and isozyme SPHK2 | Mus musculus | - |
| liver | high activity of isozyme SPHK2, low activity of isozyme SPHK1 | Mus musculus | - |
| lung | high activity of isozyme SPHK1, low activity of isozyme SPHK2 | Mus musculus | - |
| lymph node | low activity of isozyme SPHK1 and isozyme SPHK2 | Mus musculus | - |
| additional information | tissue distribution of isozyme SPHK1 and isozyme SPHK2 expression and activity, overview | Mus musculus | - |
| spleen | isozyme SPHK1, low activity of isozyme SPHK2 | Mus musculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
Vmax of different splicing variants of both isozymes | Homo sapiens |
| additional information | - |
Vmax of different splicing variants of both isozymes | Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + FTY720 | immunomodulatory drug, high activity with isozyme SPHK2, 7fold lower activity with isozyme SPHK1 | Homo sapiens | ADP + FTY720 1-phosphate | - |
? | |
| ATP + FTY720 | immunomodulatory drug, high activity with isozyme SPHK2, lower activity with isozyme SPHK1 | Mus musculus | ADP + FTY720 1-phosphate | - |
? | |
| ATP + sphinganine | - |
Homo sapiens | ADP + sphinganine 1-phosphate | - |
? | |
| ATP + sphinganine | - |
Mus musculus | ADP + sphinganine 1-phosphate | - |
? | |
| ATP + sphinganine | 2fold higher activity with isozyme SPHK1 compared to isozyme SPHK2 | Homo sapiens | ADP + sphinganine 1-phosphate | - |
? | |
| ATP + sphingosine | - |
Mus musculus | ADP + sphingosine 1-phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| additional information | enzyme belongs to the SPHK superfamily | Homo sapiens |
| additional information | enzyme belongs to the SPHK superfamily | Mus musculus |
| sphinganine kinase | - |
Homo sapiens |
| sphinganine kinase | - |
Mus musculus |
| SPHK | - |
Homo sapiens |
| SPHK | - |
Mus musculus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Homo sapiens |
| 30 | - |
assay at | Mus musculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Homo sapiens |
| 7.4 | - |
assay at | Mus musculus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | binding site contains the GGDGX20K motif | Homo sapiens | |
| ATP | - |
Mus musculus | |
| ATP | binding site contain the GGDGX20K motif | Homo sapiens | |
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