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Literature summary for 2.7.1.91 extracted from

  • Yokota, S.; Taniguchi, Y.; Kihara, A.; Mitsutake, S.; Igarashi, Y.
    Asp177 in C4 domain of mouse sphingosine kinase 1a is important for the sphingosine recognition (2004), FEBS Lett., 578, 106-110.
    View publication on PubMed
Search for more literature for 2.7.1.91

Activating Compound

Activating Compound Comment Organism Structure
Calmodulin Ca2+-dependent binding of recombinant wild-type and mutant enzymes Mus musculus

Cloned(Commentary)

Cloned (Comment) Organism
expression of FLAG-tagged isozyme SK1 splicing variant a in HEK293 cells Mus musculus

Protein Variants

Protein Variants Comment Organism
D175N site-directed mutagenesis, about 50% of wild-type enzyme activity Mus musculus
D175N/D177N site-directed mutagenesis, less than 5% of wild-type enzyme activity Mus musculus
D177N site-directed mutagenesis, less than 10% of wild-type enzyme activity Mus musculus
D177N/E179Q site-directed mutagenesis, nearly inactive mutant Mus musculus
E179Q site-directed mutagenesis, about 30% of wild-type enzyme activity Mus musculus
E181Q site-directed mutagenesis, about 30% of wild-type enzyme activity Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics, recombinant wild-type and mutant enzymes Mus musculus
0.01
-
 sphingosine pH 7.4, 37°C, recombinant wild-type enzyme and mutant E181Q Mus musculus
0.026
-
 sphingosine pH 7.4, 37°C, recombinant mutant E179Q Mus musculus
0.037
-
 sphingosine pH 7.4, 37°C, recombinant mutant D175N Mus musculus
0.063
-
 ATP pH 7.4, 37°C, recombinant mutant E179Q Mus musculus
0.072
-
 ATP pH 7.4, 37°C, recombinant wild-type enzyme Mus musculus
0.077
-
 ATP pH 7.4, 37°C, recombinant mutant D175N Mus musculus
0.088
-
 ATP pH 7.4, 37°C, recombinant mutant D177N Mus musculus
0.095
-
 ATP pH 7.4, 37°C, recombinant mutant E181Q Mus musculus
0.108
-
 sphingosine pH 7.4, 37°C, recombinant mutant D177N Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ Ca2+-dependent calmodulin binding of recombinant wild-type and mutant enzymes Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + sphingosine Mus musculus involved in diverse biological functions ADP + sphingosine 1-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus Q8CI15 isozyme SK1 splicing variant a
-

Reaction

Reaction Comment Organism Reaction ID
ATP + a sphingoid base = ADP + a sphingoid base 1-phosphate Asp177 in C4 domain of isozyme SK1a is important for sphingosine substrate recognition Mus musculus
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 Vmax of recombinant wild-type and mutant enzymes Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + sphingosine
-
 Mus musculus ADP + sphingosine 1-phosphate
-
 ?
ATP + sphingosine involved in diverse biological functions Mus musculus ADP + sphingosine 1-phosphate
-
 ?

Synonyms

Synonyms Comment Organism
SK
-
 Mus musculus
sphinganine kinase
-
 Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Mus musculus

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Mus musculus