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Literature summary for 2.7.1.90 extracted from
- Pyrophosphate-dependent phosphofructokinase from Thermoproteus tenax (2001), Methods Enzymol., 331, 54-62.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| AMP | no effect | Thermoproteus tenax |  |
| ATP | no effect | Thermoproteus tenax | |
| D-fructose 2,6-bisphosphate | no activation | Thermoproteus tenax | |
| D-glucose | no effect | Thermoproteus tenax | |
| additional information | no effect of ADP, pyruvate, citrate, phosphoenolpyruvate | Thermoproteus tenax |
General Stability
| General Stability | Organism |
|---|---|
| dialysis in absence of KCl or MgCl2 results in total loss of enzyme activity | Thermoproteus tenax |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | no inhibition | Thermoproteus tenax | |
| AMP | no inhibition | Thermoproteus tenax | |
| ATP | no inhibition | Thermoproteus tenax | |
| citrate | no inhibition | Thermoproteus tenax | |
| EDTA | - |
Thermoproteus tenax | |
| additional information | no effect of glucose, pyruvate, fructose 2,6-bisphosphate | Thermoproteus tenax | |
| phosphoenolpyruvate | no inhibition | Thermoproteus tenax | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| divalent cations | required | Thermoproteus tenax | |
| Mg2+ | requirement | Thermoproteus tenax | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37000 | - |
4 * 37000, SDS-PAGE | Thermoproteus tenax |
| 100000 | - |
gel filtration | Thermoproteus tenax |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermoproteus tenax | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 74fold, to homogeneity | Thermoproteus tenax |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.5 | - |
purified enzyme, assay temperature 50°C is not the temperature optimum | Thermoproteus tenax |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + D-fructose 6-phosphate | specific for D-fructose 6-phosphate and D-fructose 1,6-bisphosphate | Thermoproteus tenax | phosphate + D-fructose 1,6-bisphosphate | - |
r | |
| diphosphate + D-fructose 6-phosphate | specific for diphosphate as phosphor donor | Thermoproteus tenax | phosphate + D-fructose 1,6-bisphosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 37000, SDS-PAGE | Thermoproteus tenax |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PPi-PFK | - |
Thermoproteus tenax |
| pyrophosphate-dependent phosphofructokinase | - |
Thermoproteus tenax |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
assay at | Thermoproteus tenax |
| 86 | - |
- |
Thermoproteus tenax |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Thermoproteus tenax |
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Release 2023.1 (January 2023)
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