Literature summary for 2.7.1.90 extracted from

Reeves, R.E.; Lobelle-Rich, P.; Eubank, W.B.
6-Phosphofructokinase (pyrophosphate) from Entamoeba histolytica (1982), Methods Enzymol., 90, 97-102.

No PubMed abstract available

Search for more literature for 2.7.1.90

General Stability

General Stability	Organism
unstable to freezing in 20 mM imidazole buffer	Entamoeba histolytica

Inhibitors

Inhibitors	Comment	Organism	Structure
Ca2+	-	Entamoeba histolytica
Ni2+	-	Entamoeba histolytica
Zn2+	-	Entamoeba histolytica

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.014	0.017	diphosphate	-	Entamoeba histolytica
0.018	0.023	D-fructose 1,6-bisphosphate	-	Entamoeba histolytica
0.038	-	D-fructose 6-phosphate	-	Entamoeba histolytica
0.06	-	sedoheptulose 7-phosphate	-	Entamoeba histolytica
0.59	1	phosphate	-	Entamoeba histolytica
3.6	-	arsenate	with D-fructose 1,6-bisphosphate	Entamoeba histolytica

Metals/Ions

Metals/Ions	Comment	Organism	Structure
divalent cations	required	Entamoeba histolytica
divalent cations	Mn2+ or Mg2+	Entamoeba histolytica
Mg2+	requirement	Entamoeba histolytica

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
83000	-	gel filtration	Entamoeba histolytica

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
diphosphate + D-fructose 6-phosphate	Entamoeba histolytica	-	phosphate + D-fructose 1,6-bisphosphate	-	?
diphosphate + D-fructose 6-phosphate	Entamoeba histolytica K-9 / ATCC 30015	-	phosphate + D-fructose 1,6-bisphosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Entamoeba histolytica	-	-	-
Entamoeba histolytica K-9 / ATCC 30015	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Entamoeba histolytica

Storage Stability

Storage Stability	Organism
-20°C, in salt-containing solution, 25% glycerol, t1/2: 1 year	Entamoeba histolytica

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
arsenate + D-fructose 1,6-bisphosphate	-	Entamoeba histolytica	?	-	r
arsenate + D-fructose 1,6-bisphosphate	-	Entamoeba histolytica K-9 / ATCC 30015	?	-	r
diphosphate + 2,5-anhydro-D-mannitol 6-phosphate	-	Entamoeba histolytica	phosphate + 2,5-anhydro-D-mannitol 1,6-bisphosphate	-	r
diphosphate + 2,5-anhydro-D-mannitol 6-phosphate	-	Entamoeba histolytica K-9 / ATCC 30015	phosphate + 2,5-anhydro-D-mannitol 1,6-bisphosphate	-	r
diphosphate + D-fructose 6-phosphate	-	Entamoeba histolytica	phosphate + D-fructose 1,6-bisphosphate	-	?
diphosphate + D-fructose 6-phosphate	specific for diphosphate as phosphor donor	Entamoeba histolytica	phosphate + D-fructose 1,6-bisphosphate	-	r
diphosphate + D-fructose 6-phosphate	-	Entamoeba histolytica K-9 / ATCC 30015	phosphate + D-fructose 1,6-bisphosphate	-	?
diphosphate + D-fructose 6-phosphate	specific for diphosphate as phosphor donor	Entamoeba histolytica K-9 / ATCC 30015	phosphate + D-fructose 1,6-bisphosphate	-	r
diphosphate + D-sedoheptulose 7-phosphate	-	Entamoeba histolytica	phosphate + D-sedoheptulose 1,7-bisphosphate	-	r
diphosphate + D-sedoheptulose 7-phosphate	-	Entamoeba histolytica K-9 / ATCC 30015	phosphate + D-sedoheptulose 1,7-bisphosphate	-	r

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
additional information	-	additional information	-	Entamoeba histolytica

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Release 2023.1 (January 2023)