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Literature summary for 2.7.1.90 extracted from
- Properties of pyrophosphate:D-fructose-6-phosphate phosphotransferase from endosperm of developing wheat (Triticum aestivum L.) grains (1989), Plant Physiol., 91, 421-426.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| D-fructose 2,6-bisphosphate | - |
Triticum aestivum |  |
General Stability
| General Stability | Organism |
|---|---|
| ethylene glycol stabilizes during purification, almost complete loss of activity without | Triticum aestivum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| bicarbonate | - |
Triticum aestivum | |
| Cl- | - |
Triticum aestivum | |
| additional information | no inhibition by acetate, carbonate | Triticum aestivum | |
| NO3- | no inhibition | Triticum aestivum | |
| phosphate | in the forward reaction only in presence of fructose 2,6-bisphosphate | Triticum aestivum | |
| SO42- | no inhibition | Triticum aestivum | |
| sulfite | - |
Triticum aestivum | |
| thiosulfate | - |
Triticum aestivum | |
| tungstate | - |
Triticum aestivum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.025 | 0.033 | diphosphate | - |
Triticum aestivum | |
| 0.129 | - |
phosphate | - |
Triticum aestivum | |
| 0.139 | 0.21 | D-fructose 1,6-bisphosphate | - |
Triticum aestivum | |
| 0.3 | 0.38 | D-fructose 6-phosphate | - |
Triticum aestivum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | no activation of forward reaction | Triticum aestivum | |
| Co2+ | no activitaion | Triticum aestivum | |
| Mg2+ | requirement | Triticum aestivum | |
| additional information | no activation by Cu2+ | Triticum aestivum | |
| additional information | no activation by Ba2+, Sn2+, Sr2+ | Triticum aestivum |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 80000 | - |
1 * 80000 + 1 * 90000, SDS-PAGE | Triticum aestivum |
| 90000 | - |
1 * 80000 + 1 * 90000, SDS-PAGE | Triticum aestivum |
| 170000 | - |
gel filtration | Triticum aestivum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + D-fructose 6-phosphate | Triticum aestivum | - |
phosphate + D-fructose 1,6-bisphosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Triticum aestivum | - |
cv. WH-157 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial, 127fold | Triticum aestivum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| endosperm | - |
Triticum aestivum | - |
| seed | germinating | Triticum aestivum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.5 | - |
partially purified enzyme | Triticum aestivum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, in 50 mM HEPES buffer, pH 7.5, 10 mM 2-mercaptoethanol, 0.5 mM EDTA, 1 mM MgCl2, 5 mM KCl, 30% ethylene glycol, more than 1 month | Triticum aestivum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + D-fructose 6-phosphate | - |
Triticum aestivum | phosphate + D-fructose 1,6-bisphosphate | - |
r | |
| diphosphate + D-fructose 6-phosphate | specific for diphosphate as phosphor donor | Triticum aestivum | phosphate + D-fructose 1,6-bisphosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 80000 + 1 * 90000, SDS-PAGE | Triticum aestivum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PFP | - |
Triticum aestivum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
15 min stable | Triticum aestivum |
| 50 | - |
t1/2: 15 min | Triticum aestivum |
| 60 | - |
above, complete inactivation | Triticum aestivum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
forward reaction, D-fructose 2,6-bisphosphate has no effect on pH-optimum | Triticum aestivum |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.78 | - |
phosphate | versus fructose 6-phosphate, pH 7.5, 25°C, in presence of fructose 2,6-bisphosphate at saturating concentration | Triticum aestivum | |
| 1.2 | - |
phosphate | versus diphosphate, pH 7.5, 25°C, in presence of fructose 2,6-bisphosphate at saturating concentration | Triticum aestivum | |
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