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Literature summary for 2.7.1.90 extracted from
- A novel pyrophosphate D-fructose-6-phosphate 1-phosphotransferase from carrot roots. Relation to PFK from the same source (1988), FEBS Lett., 238, 405-410.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| D-fructose 2,6-bisphosphate | forward reaction only | Daucus carota |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetic parameters, in the absence or presence of D-fructose 2,6-bisphosphate | Daucus carota | |
| 0.014 | 0.017 | diphosphate | pH 8.0, 25°C | Daucus carota | |
| 0.13 | - |
D-fructose 6-phosphate | pH 8.0, 25°C | Daucus carota | |
| 0.5 | - |
Mg2+ | forward reaction, pH 8.0, 25°C | Daucus carota | |
| 1 | - |
D-fructose 1,6-bisphosphate | reverse reaction, pH 8.0, 25°C | Daucus carota | |
| 1.7 | - |
phosphate | reverse reaction, pH 8.0, 25°C | Daucus carota | |
| 1.9 | - |
Mg2+ | reverse reaction, pH 8.0, 25°C | Daucus carota | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | requirement | Daucus carota | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
2 * 60000 + 2 * 65000-67000, alpha2-beta2, SDS-PAGE | Daucus carota |
| 294000 | - |
gel filtration, enzyme does not dissociate in the presence of D-fructose 2,6-bisphosphate | Daucus carota |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + D-fructose 6-phosphate | Daucus carota | - |
phosphate + D-fructose 1,6-bisphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Daucus carota | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 167fold | Daucus carota |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| root | - |
Daucus carota | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2 | - |
purified enzyme | Daucus carota |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + D-fructose 6-phosphate | - |
Daucus carota | phosphate + D-fructose 1,6-bisphosphate | - |
? | |
| diphosphate + D-fructose 6-phosphate | ratio forward/reverse reaction: 7:1 without D-fructose 2,6-bisphosphate and 1:1 in the presence of this effector | Daucus carota | phosphate + D-fructose 1,6-bisphosphate | - |
r | |
| diphosphate + D-fructose 6-phosphate | specific for diphosphate as phosphor donor | Daucus carota | phosphate + D-fructose 1,6-bisphosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 2 * 60000 + 2 * 65000-67000, alpha2-beta2, SDS-PAGE | Daucus carota |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Daucus carota |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Daucus carota |
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Release 2023.1 (January 2023)
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