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Literature summary for 2.7.1.90 extracted from
- 6-Phosphofructokinase (pyrophosphate). Properties of the enzyme from Entamoeba histolytica and its reaction mechanism (1976), J. Biol. Chem., 251, 2958-2962.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | weak | Entamoeba histolytica |  |
| Ca2+ | - |
Entamoeba histolytica | |
| D-fructose 6-phosphate | competitive versus D-fructose 1,6-bisphosphate and phosphate | Entamoeba histolytica | |
| diphosphate | - |
Entamoeba histolytica | |
| EDTA | - |
Entamoeba histolytica | |
| Ni2+ | - |
Entamoeba histolytica | |
| phosphate | competitive versus diphosphate | Entamoeba histolytica | |
| Zn2+ | - |
Entamoeba histolytica | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.006 | - |
diphosphate | pH 7.0 | Entamoeba histolytica | |
| 0.008 | - |
Mg2+ | forward reaction, pH 7.0, 30°C | Entamoeba histolytica | |
| 0.009 | - |
D-fructose 1,6-bisphosphate | pH 7.0, 30°C | Entamoeba histolytica | |
| 0.5 | - |
Mg2+ | reverse reaction, pH 7.0, 30°C | Entamoeba histolytica | |
| 0.59 | 1 | phosphate | - |
Entamoeba histolytica | |
| 3.6 | - |
arsenate | pH 7.0, 30°C | Entamoeba histolytica | |
| 3.6 | - |
arsenate | with D-fructose 1,6-bisphosphate | Entamoeba histolytica | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | less effective than Mg2+ | Entamoeba histolytica | |
| Co2+ | less effective than Mn2+ | Entamoeba histolytica | |
| divalent cations | required | Entamoeba histolytica | |
| divalent cations | desecending order Mn2+, Mg2+, Co2+ | Entamoeba histolytica | |
| Mg2+ | requirement | Entamoeba histolytica | |
| Mg2+ | less effective than Mn2+ | Entamoeba histolytica | |
| Mg2+ | enzyme requires 0.5 mM in the forward reaction, 0.0008 mM in the reverse reaction | Entamoeba histolytica | |
| Mn2+ | requirement | Entamoeba histolytica | |
| additional information | no activation by Ni2+ | Entamoeba histolytica | |
| additional information | no activation by Zn2+ | Entamoeba histolytica |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 83000 | - |
gel filtration | Entamoeba histolytica |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + D-fructose 6-phosphate | Entamoeba histolytica | - |
phosphate + D-fructose 1,6-bisphosphate | - |
? | |
| diphosphate + D-fructose 6-phosphate | Entamoeba histolytica DKB | - |
phosphate + D-fructose 1,6-bisphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Entamoeba histolytica | - |
DKB-strain | - |
| Entamoeba histolytica DKB | - |
DKB-strain | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Entamoeba histolytica |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| diphosphate + D-fructose 6-phosphate = phosphate + D-fructose 1,6-bisphosphate | random bi bi mechanism | Entamoeba histolytica |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 40 | - |
partially purified enzyme | Entamoeba histolytica |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, partially purified enzyme, loss of 25% activity after 1 month | Entamoeba histolytica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| arsenate + D-fructose 1,6-bisphosphate | - |
Entamoeba histolytica | ? | - |
r | |
| arsenate + D-fructose 1,6-bisphosphate | - |
Entamoeba histolytica DKB | ? | - |
r | |
| diphosphate + D-fructose 6-phosphate | - |
Entamoeba histolytica | phosphate + D-fructose 1,6-bisphosphate | - |
? | |
| diphosphate + D-fructose 6-phosphate | no phosphate group exchange reaction between phosphate and diphosphate, or D-fructose 6-phosphate and D-fructose 1,6-bisphosphate in absence of a third substrate | Entamoeba histolytica | phosphate + D-fructose 1,6-bisphosphate | arsenate can replace phosphate in the reverse reaction | r | |
| diphosphate + D-fructose 6-phosphate | specific for diphosphate as phosphor donor | Entamoeba histolytica | phosphate + D-fructose 1,6-bisphosphate | arsenate can replace phosphate in the reverse reaction | r | |
| diphosphate + D-fructose 6-phosphate | - |
Entamoeba histolytica DKB | phosphate + D-fructose 1,6-bisphosphate | - |
? | |
| diphosphate + D-fructose 6-phosphate | no phosphate group exchange reaction between phosphate and diphosphate, or D-fructose 6-phosphate and D-fructose 1,6-bisphosphate in absence of a third substrate | Entamoeba histolytica DKB | phosphate + D-fructose 1,6-bisphosphate | arsenate can replace phosphate in the reverse reaction | r | |
| diphosphate + D-fructose 6-phosphate | specific for diphosphate as phosphor donor | Entamoeba histolytica DKB | phosphate + D-fructose 1,6-bisphosphate | arsenate can replace phosphate in the reverse reaction | r | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Entamoeba histolytica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Entamoeba histolytica |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.115 | - |
D-fructose 6-phosphate | versus diphosphate, pH 7.0, 30°C | Entamoeba histolytica | |
| 0.16 | - |
D-fructose 6-phosphate | versus D-fructose 1,6-bisphosphate, pH 7.0, 30°C | Entamoeba histolytica | |
| 0.85 | - |
phosphate | versus diphosphate, pH 7.0, 30°C | Entamoeba histolytica | |
| 3 | - |
phosphate | versus D-fructose 1,6-bisphosphate, pH 7.0, 30°C | Entamoeba histolytica | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Entamoeba histolytica | - |
- |
5.8 |
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