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Literature summary for 2.7.1.90 extracted from

  • Reeves, R.E.; South, D.J.; Blytt, H.J.; Warren, L.G.
    Pyrophosphate:D-fructose 6-phosphate 1-phosphotransferase. A new enzyme with the glycolytic function of 6-phosphofructokinase (1974), J. Biol. Chem., 249, 7737-7741.
    View publication on PubMed

General Stability

General Stability Organism
enzyme is stable if whole cells are lyophilized or vigorously sonicated Entamoeba histolytica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.014 0.017 diphosphate
-
Entamoeba histolytica
0.018 0.023 D-fructose 1,6-bisphosphate
-
Entamoeba histolytica
0.038
-
D-fructose 6-phosphate
-
Entamoeba histolytica
0.59 1 phosphate
-
Entamoeba histolytica

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Entamoeba histolytica 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ requirement Entamoeba histolytica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
diphosphate + D-fructose 6-phosphate Entamoeba histolytica
-
phosphate + D-fructose 1,6-bisphosphate
-
?
diphosphate + D-fructose 6-phosphate Entamoeba histolytica H200
-
phosphate + D-fructose 1,6-bisphosphate
-
?

Organism

Organism UniProt Comment Textmining
Entamoeba histolytica
-
-
-
Entamoeba histolytica H200
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Entamoeba histolytica

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
45.7
-
purified enzyme Entamoeba histolytica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphosphate + D-fructose 6-phosphate
-
Entamoeba histolytica phosphate + D-fructose 1,6-bisphosphate
-
?
diphosphate + D-fructose 6-phosphate specific for diphosphate as phosphor donor Entamoeba histolytica phosphate + D-fructose 1,6-bisphosphate
-
?
diphosphate + D-fructose 6-phosphate
-
Entamoeba histolytica H200 phosphate + D-fructose 1,6-bisphosphate
-
?
diphosphate + D-fructose 6-phosphate specific for diphosphate as phosphor donor Entamoeba histolytica H200 phosphate + D-fructose 1,6-bisphosphate
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
21 22 assay at Entamoeba histolytica