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Literature summary for 2.7.1.86 extracted from
- Characterization of NADH kinase from Saccharomyces cerevisiae (1989), J. Biochem., 105, 588-593.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| acetate | activates | Saccharomyces cerevisiae |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3-acetyl-NAD+ | 1 mM, 15% inhibition | Saccharomyces cerevisiae | |
| ATP | above 6 mM | Saccharomyces cerevisiae | |
| iodoacetic acid | 5 mM, 87% inhibition, NADH protects | Saccharomyces cerevisiae | |
| NEM | 5 mM, 77% inhibition, NADH protects, ATP not | Saccharomyces cerevisiae | |
| PCMB | 5 mM, 49% inhibition | Saccharomyces cerevisiae | |
| phosphoenolpyruvate | 30 mM, 57% inhibition | Saccharomyces cerevisiae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.105 | - |
NADH | pH 7.4, 30°C | Saccharomyces cerevisiae | |
| 2.1 | - |
ATP | pH 7.4, 30°C | Saccharomyces cerevisiae | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | localized exclusively in mitochondria | Saccharomyces cerevisiae | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 160000 | - |
gel filtration | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
| Saccharomyces cerevisiae X2181-1A | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 729 | - |
- |
Saccharomyces cerevisiae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -30°C, 20 mM Tris-HCl buffer, pH 7.8, 0.2 M ammonium sulfate, 20 mM MgCl2, 2 mM EDTA, stable for 1 month or more | Saccharomyces cerevisiae |
| 0-5°C, 20 mM Tris-HCl buffer, pH 7.8, 0.2 M ammonium sulfate, 20 mM MgCl2, 2 mM EDTA, stable for 7 days | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + NADH | specific for NADH | Saccharomyces cerevisiae | ADP + NADPH | - |
? | |
| ATP + NADH | NAD+ completely ineffective | Saccharomyces cerevisiae | ADP + NADPH | - |
? | |
| ATP + NADH | specific for NADH | Saccharomyces cerevisiae X2181-1A | ADP + NADPH | - |
? | |
| ATP + NADH | NAD+ completely ineffective | Saccharomyces cerevisiae X2181-1A | ADP + NADPH | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | presence of 2 or more subunits of different size | Saccharomyces cerevisiae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
5 min, 35% loss of activity | Saccharomyces cerevisiae |
| 55 | - |
5 min, 95% loss of activity | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Saccharomyces cerevisiae |
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Release 2023.1 (January 2023)
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