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Literature summary for 2.7.1.83 extracted from
- A kinase and a glycosylase catabolize pseudouridine in the peroxisome to prevent toxic pseudouridine monophosphate accumulation (2020), Plant Cell, 32, 722-739 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Nicotiana benthamiana leaves | Arabidopsis thaliana |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0044 | - |
pseudouridine | at pH 7.5 and 30°C | Arabidopsis thaliana |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| peroxisome | - |
Arabidopsis thaliana | 5777 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | 2 mM used in assay conditions | Arabidopsis thaliana | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + pseudouridine | Arabidopsis thaliana | - |
ADP + pseudouridine 5'-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| strep-tactin affinity column chromatography | Arabidopsis thaliana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + pseudouridine | - |
Arabidopsis thaliana | ADP + pseudouridine 5'-phosphate | - |
? | |
| additional information | no activity with adenosine, guanosine, inosine, cytidine, uridine and thymidine | Arabidopsis thaliana | ? | - |
- |
|
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 41000, Strep-tagged enzyme, SDS-PAGE | Arabidopsis thaliana |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PUKI | - |
Arabidopsis thaliana |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.23 | - |
pseudouridine | at pH 7.5 and 30°C | Arabidopsis thaliana | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 52.3 | - |
pseudouridine | at pH 7.5 and 30°C | Arabidopsis thaliana | |
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Release 2023.1 (January 2023)
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