Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 50% inhibition at 1 mM | Propionibacterium freudenreichii subsp. shermanii | |
Cd2+ | 65% inhibition at 1 mM | Propionibacterium freudenreichii subsp. shermanii | |
D-serine | 20 mM, weak inhibition | Propionibacterium freudenreichii subsp. shermanii | |
DL-homoserine | 100 mM, weak inhibition | Propionibacterium freudenreichii subsp. shermanii | |
EDTA | inpresence of phosphate | Propionibacterium freudenreichii subsp. shermanii | |
glycerol | 100 mM, weak inhibition | Propionibacterium freudenreichii subsp. shermanii | |
glycine | 20% inhibition at 10 mM | Propionibacterium freudenreichii subsp. shermanii | |
Glycyl-L-serine | weak inhibition | Propionibacterium freudenreichii subsp. shermanii | |
Hg2+ | 55% inhibition at 1 mM | Propionibacterium freudenreichii subsp. shermanii | |
L-alanine | 35% inhibition at 10 mM | Propionibacterium freudenreichii subsp. shermanii | |
L-threonine | 100 mM, weak inhibition | Propionibacterium freudenreichii subsp. shermanii | |
La3+ | 25% inhibition at 1 mM | Propionibacterium freudenreichii subsp. shermanii | |
phosphate | competitive | Propionibacterium freudenreichii subsp. shermanii | |
Sr2+ | weak inhibition | Propionibacterium freudenreichii subsp. shermanii | |
Tris | 0.6 M, weak inhibition | Propionibacterium freudenreichii subsp. shermanii | |
Zn2+ | 85% inhibition at 10 mM | Propionibacterium freudenreichii subsp. shermanii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
diphosphate | pH 7.8, 37°C | Propionibacterium freudenreichii subsp. shermanii | |
0.13 | - |
Mg2+ | 1 mM diphosphate, pH 7.8, 37°C | Propionibacterium freudenreichii subsp. shermanii | |
1.9 | - |
L-serine | pH 7.8, 37°C | Propionibacterium freudenreichii subsp. shermanii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | slight stimulation, about 14% of activity compared to Mg2+ | Propionibacterium freudenreichii subsp. shermanii | |
Co2+ | slight stimulation, about 42% of activity compared to Mg2+ | Propionibacterium freudenreichii subsp. shermanii | |
Cu2+ | slight stimulation, about 14% of activity compared to Mg2+ | Propionibacterium freudenreichii subsp. shermanii | |
Mg2+ | high stimulation | Propionibacterium freudenreichii subsp. shermanii | |
Mn2+ | slight stimulation, about 34% of activity compared to Mg2+ | Propionibacterium freudenreichii subsp. shermanii | |
Zn2+ | slight stimulation, about 14% of activity compared to Mg2+ | Propionibacterium freudenreichii subsp. shermanii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65000 | - |
gel filtration | Propionibacterium freudenreichii subsp. shermanii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + L-serine | Propionibacterium freudenreichii subsp. shermanii | - |
phosphate + O-phospho-L-serine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Propionibacterium freudenreichii subsp. shermanii | - |
strain ATCC 9614 | - |
Purification (Comment) | Organism |
---|---|
partial, 102fold | Propionibacterium freudenreichii subsp. shermanii |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
diphosphate + L-serine = phosphate + O-phospho-L-serine | binary or ping-pong mechanism | Propionibacterium freudenreichii subsp. shermanii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
9.5 | - |
partially purified enzyme | Propionibacterium freudenreichii subsp. shermanii |
Storage Stability | Organism |
---|---|
4°C o r-15°C, 0.02 M Tris buffer, pH 7.8, stable for several weeks | Propionibacterium freudenreichii subsp. shermanii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + alpha-methylserine | 7% activity compared to L-serine | Propionibacterium freudenreichii subsp. shermanii | phosphate + alpha-methyl-O-phospho-L-serine | - |
? | |
diphosphate + DL-homoserine | 7% activity compared to L-serine | Propionibacterium freudenreichii subsp. shermanii | phosphate + O-phospho-DL-homoserine | - |
? | |
diphosphate + glycyl-L-serine | 16% activity compared to L-serine | Propionibacterium freudenreichii subsp. shermanii | phosphate + glycyl-O-phospho-L-serine | - |
? | |
diphosphate + L-serine | - |
Propionibacterium freudenreichii subsp. shermanii | phosphate + O-phospho-L-serine | - |
? | |
diphosphate + L-threonine | 8% activity compared to L-serine | Propionibacterium freudenreichii subsp. shermanii | phosphate + O-phospho-L-threonine | - |
? | |
diphosphate + N-chloroacetyl-L-serine | 19% activity compared to L-serine | Propionibacterium freudenreichii subsp. shermanii | phosphate + N-chloroacetyl-O-phospho-L-serine | - |
? | |
additional information | substrate specificity | Propionibacterium freudenreichii subsp. shermanii | ? | - |
? | |
additional information | no activity with D-serine and L-cysteine, tripolyphosphate can substitute to some extent for diphosphate | Propionibacterium freudenreichii subsp. shermanii | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Propionibacterium freudenreichii subsp. shermanii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
stable at least 12 h | Propionibacterium freudenreichii subsp. shermanii |
37 | - |
stable at least 1 h | Propionibacterium freudenreichii subsp. shermanii |
65 | - |
15 min, 46% remaining activity | Propionibacterium freudenreichii subsp. shermanii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Propionibacterium freudenreichii subsp. shermanii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.4 | 7.8 | 60% of maximal activity at pH 5.4 and pH 7.8 | Propionibacterium freudenreichii subsp. shermanii |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.8 | - |
glycine | pH 7.8, 37°C | Propionibacterium freudenreichii subsp. shermanii |