Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Acetivibrio thermocellus |
Crystallization (Comment) | Organism |
---|---|
in complexes with GTP, CTP, UTP and dATP, hanging drop vapor diffusion method, using 100 mM sodium citrate, pH 5.6, 100 mM MgCl2, 16-18% (w/v) PEG-3350 | Acetivibrio thermocellus |
Protein Variants | Comment | Organism |
---|---|---|
R116A | the mutation elicits a 10fold increase in Km for ATP, but has little effect on the turnover number value leading to 83% of wild type activity | Acetivibrio thermocellus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0028 | - |
5'-HO-CCGACCAACGAAGGT | wild type enzyme, at pH 7.0 and 37°C | Acetivibrio thermocellus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Acetivibrio thermocellus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5'-dephospho-DNA | Acetivibrio thermocellus | - |
ADP + 5'-phospho-DNA | - |
r | |
ATP + 5'-dephospho-RNA | Acetivibrio thermocellus | - |
ADP + 5'-phospho-RNA | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetivibrio thermocellus | A3DJ38 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-agarose column chromatography, and gel filtration | Acetivibrio thermocellus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + 5'-HO-AUCACGCUUpCp | - |
Acetivibrio thermocellus | ? | - |
r | |
ATP + 5'-dephospho-DNA | - |
Acetivibrio thermocellus | ADP + 5'-phospho-DNA | - |
r | |
ATP + 5'-dephospho-RNA | - |
Acetivibrio thermocellus | ADP + 5'-phospho-RNA | - |
r | |
ATP + 5'-HO-CCGACCAACGAAGGT | - |
Acetivibrio thermocellus | ? | - |
r | |
CTP + 5'-dephospho-RNA | - |
Acetivibrio thermocellus | CDP + 5'-phospho-RNA | - |
r | |
dATP + 5'-dephospho-RNA | - |
Acetivibrio thermocellus | dADP + 5'-phospho-RNA | - |
r | |
GTP + 5'-dephospho-RNA | - |
Acetivibrio thermocellus | GDP + 5'-phospho-RNA | - |
r | |
UTP + 5'-dephospho-RNA | - |
Acetivibrio thermocellus | UDP + 5'-phospho-RNA | - |
r |
Synonyms | Comment | Organism |
---|---|---|
PNKP | the enzyme is composed of three catalytic modules: an N-terminal 5'-OH polynucleotide kinase, a central 2',3' phosphatase, and a C-terminal ligase polynucleotide kinase | Acetivibrio thermocellus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.072 | - |
5'-HO-CCGACCAACGAAGGT | wild type enzyme, at pH 7.0 and 37°C | Acetivibrio thermocellus |