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Literature summary for 2.7.1.78 extracted from
- Mechanism of RNA 2,3-cyclic phosphate end healing by T4 polynucleotide kinase-phosphatase (2013), Nucleic Acids Res., 41, 355-365.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Tequatrovirus T4 |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 5'-dephospho-DNA | Tequatrovirus T4 | - |
ADP + 5'-phospho-DNA | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Tequatrovirus T4 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-agarose column chromatography, and gel filtration | Tequatrovirus T4 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 5'-dephospho-DNA | - |
Tequatrovirus T4 | ADP + 5'-phospho-DNA | - |
? | |
| additional information | the enzyme also has RNA 2'-phosphatase activity that requires Asp165 and Asp167 | Tequatrovirus T4 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PNKP | - |
Tequatrovirus T4 |
| polynucleotide kinase-phosphatase | - |
Tequatrovirus T4 |
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Release 2023.1 (January 2023)
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