Cloned (Comment) | Organism |
---|---|
into the vector pET28-His10Smt3 for expression in Escherichia coli BL21DE3 cells | Pyrococcus horikoshii |
Protein Variants | Comment | Organism |
---|---|---|
D73A | mutation abolishes activity | Pyrococcus horikoshii |
K49A | mutation reduces activity to 1% of the wild-type value | Pyrococcus horikoshii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.016 | - |
5'-OH DNA | - |
Pyrococcus horikoshii | |
0.021 | - |
5'-phospho-DNA | - |
Pyrococcus horikoshii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Pyrococcus horikoshii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41000 | - |
determined by SDS-PAGE, tag-free | Pyrococcus horikoshii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5'-OH DNA | Pyrococcus horikoshii | - |
ADP + 5'-phospho-DNA | - |
r | |
ATP + 5'-OH RNA | Pyrococcus horikoshii | preference for RNA substrates | ADP + 5'-phospho-RNA | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus horikoshii | Q57936 | - |
- |
Purification (Comment) | Organism |
---|---|
on a Ni-NTA-agarose column, the His10Smt3 tag is removed with the Smt3-specific protease Ulp1 | Pyrococcus horikoshii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5'-OH DNA | - |
Pyrococcus horikoshii | ADP + 5'-phospho-DNA | - |
r | |
ATP + 5'-OH RNA | preference for RNA substrates | Pyrococcus horikoshii | ADP + 5'-phospho-RNA | - |
r |
Synonyms | Comment | Organism |
---|---|---|
5'-OH polynucleotide kinase | - |
Pyrococcus horikoshii |
Clp1-like protein | - |
Pyrococcus horikoshii |
PhoClp1 | - |
Pyrococcus horikoshii |
polynucleotide kinase | - |
Pyrococcus horikoshii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
kinase assay | Pyrococcus horikoshii |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | 85 | - |
Pyrococcus horikoshii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00083 | - |
5'-phospho-DNA | - |
Pyrococcus horikoshii | |
0.077 | - |
5'-OH DNA | - |
Pyrococcus horikoshii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
reverse reaction | Pyrococcus horikoshii |
6.5 | - |
kinase assay | Pyrococcus horikoshii |
7.5 | - |
kinase assay | Pyrococcus horikoshii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 9.5 | DNA kinase activity | Pyrococcus horikoshii |
4.5 | 6.5 | RNA kinase activity | Pyrococcus horikoshii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Pyrococcus horikoshii |