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show all sequences of 2.7.1.78

Real-time monitoring of the activity and kinetics of T4 polynucleotide kinase by a singly labeled DNA-hairpin smart probe coupled with lambda exonuclease cleavage

Song, C.; Zhao, M.; Anal. Chem. 81, 1383-1388 (2009)

Data extracted from this reference:

Application
Application
Commentary
Organism
analysis
a method for real-time monitoring of the activity and kinetics of T4 polynucleotide kinase by use of a singly fluorophore-labeled DNA-hairpin smart probe coupled with lambda exonuclease cleavage is described
Escherichia virus T4
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00056
-
GGGCC(AG)10GGCCC-fluorescein
-
Escherichia virus T4
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + 5'-dephospho-DNA
Escherichia virus T4
-
ADP + 5'-phospho-DNA
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia virus T4
P06855
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + (AG)10GGCCC-fluorescein
-
695561
Escherichia virus T4
?
-
-
-
?
ATP + (AG)10GGCCC-tetramethylrhodamine
-
695561
Escherichia virus T4
?
-
-
-
?
ATP + 5'-dephospho-DNA
-
695561
Escherichia virus T4
ADP + 5'-phospho-DNA
-
-
-
?
ATP + GGGCC(AG)10GGCCC-fluorescein
-
695561
Escherichia virus T4
?
-
-
-
?
ATP + GGGCC(AG)10GGCCC-tetramethylrhodamine
-
695561
Escherichia virus T4
?
-
-
-
?
ATP + GGGCC(AG)12GGCCC-fluorescein
-
695561
Escherichia virus T4
?
-
-
-
?
ATP + GGGCC(AG)8GGCCC-fluorescein
-
695561
Escherichia virus T4
?
-
-
-
?
ATP + GGGGC(AG)10GCCCC-fluorescein
-
695561
Escherichia virus T4
?
-
-
-
?
ATP + GGGGC(AG)10GCCCC-tetramethylrhodamine
-
695561
Escherichia virus T4
?
-
-
-
?
ATP + GGGGG(AG)10CCCCC-fluorescein
-
695561
Escherichia virus T4
?
-
-
-
?
ATP + GGGGG(AG)10CCCCC-tetramethylrhodamine
-
695561
Escherichia virus T4
?
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
activity assay
Escherichia virus T4
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
198
-
GGGCC(AG)10GGCCC-fluorescein
-
Escherichia virus T4
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
activity assay
Escherichia virus T4
Cofactor
Cofactor
Commentary
Organism
Structure
ATP
-
Escherichia virus T4
Application (protein specific)
Application
Commentary
Organism
analysis
a method for real-time monitoring of the activity and kinetics of T4 polynucleotide kinase by use of a singly fluorophore-labeled DNA-hairpin smart probe coupled with lambda exonuclease cleavage is described
Escherichia virus T4
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
ATP
-
Escherichia virus T4
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00056
-
GGGCC(AG)10GGCCC-fluorescein
-
Escherichia virus T4
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + 5'-dephospho-DNA
Escherichia virus T4
-
ADP + 5'-phospho-DNA
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + (AG)10GGCCC-fluorescein
-
695561
Escherichia virus T4
?
-
-
-
?
ATP + (AG)10GGCCC-tetramethylrhodamine
-
695561
Escherichia virus T4
?
-
-
-
?
ATP + 5'-dephospho-DNA
-
695561
Escherichia virus T4
ADP + 5'-phospho-DNA
-
-
-
?
ATP + GGGCC(AG)10GGCCC-fluorescein
-
695561
Escherichia virus T4
?
-
-
-
?
ATP + GGGCC(AG)10GGCCC-tetramethylrhodamine
-
695561
Escherichia virus T4
?
-
-
-
?
ATP + GGGCC(AG)12GGCCC-fluorescein
-
695561
Escherichia virus T4
?
-
-
-
?
ATP + GGGCC(AG)8GGCCC-fluorescein
-
695561
Escherichia virus T4
?
-
-
-
?
ATP + GGGGC(AG)10GCCCC-fluorescein
-
695561
Escherichia virus T4
?
-
-
-
?
ATP + GGGGC(AG)10GCCCC-tetramethylrhodamine
-
695561
Escherichia virus T4
?
-
-
-
?
ATP + GGGGG(AG)10CCCCC-fluorescein
-
695561
Escherichia virus T4
?
-
-
-
?
ATP + GGGGG(AG)10CCCCC-tetramethylrhodamine
-
695561
Escherichia virus T4
?
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
activity assay
Escherichia virus T4
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
198
-
GGGCC(AG)10GGCCC-fluorescein
-
Escherichia virus T4
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
activity assay
Escherichia virus T4
Other publictions for EC 2.7.1.78
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
751515
Zhang
Determination of the activity ...
Escherichia virus T4
Microchim. Acta
186
003
2019
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1
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749573
Ma
-
A fluorescence-based assay fo ...
Escherichia virus T4
Anal. Methods
8
1989-1994
2016
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1
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1
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1
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737436
Tao
Highly specific fluorescence d ...
Escherichia virus T4
Anal. Biochem.
485
18-24
2015
-
-
-
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-
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3
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1
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737444
Zhou
One-strand oligonucleotide pro ...
Escherichia virus T4
Analyst
140
5650-5655
2015
-
-
-
-
-
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3
-
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1
-
1
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737890
Lian
Detection of T4 polynucleotide ...
Escherichia virus T4
Biosens. Bioelectron.
66
316-320
2015
-
-
-
-
-
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3
-
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1
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1
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750414
Shimada
Polynucleotide kinase-phospha ...
Mus musculus
EMBO J.
34
2465-2480
2015
-
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1
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751906
Chatterjee
The role of the mammalian DNA ...
Homo sapiens
PLoS Genet.
11
e1004749
2015
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1
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1
1
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752288
Dong
A label-free assay for T4 pol ...
Escherichia virus T4
Talanta
146
253-258
2015
-
1
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729562
Karaca
Human CLP1 mutations alter tRN ...
Homo sapiens
Cell
157
636-650
2014
-
-
-
-
2
-
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1
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1
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1
1
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729563
Schaffer
CLP1 founder mutation links tR ...
Homo sapiens
Cell
157
651-663
2014
-
-
-
-
1
-
-
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1
1
-
3
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1
1
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1
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1
1
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737441
Hou
Amplified detection of T4 poly ...
Escherichia virus T4
Anal. Chem.
86
884-890
2014
-
-
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-
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1
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1
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737443
Wang
Detection of polynucleotide ki ...
Escherichia virus T4
Analyst
139
3895-3900
2014
-
-
-
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3
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1
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737575
Strazdaite-Zieliene
A mutation in the gene for pol ...
Escherichia virus T4, Escherichia virus T4 K10
Arch. Virol.
159
327-331
2014
-
-
1
-
2
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6
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3
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1
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6
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1
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2
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6
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1
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6
-
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738535
Das
Structures of bacterial polynu ...
Hungateiclostridium thermocellum, Hungateiclostridium thermocellum DSM 1237
J. Bacteriol.
196
4285-4292
2014
-
-
1
1
4
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1
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2
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3
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1
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2
1
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4
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2
1
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739067
Dikfidan
RNA specificity and regulation ...
Caenorhabditis elegans
Mol. Cell
54
975-986
2014
-
-
1
1
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3
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2
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3
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729174
Mair
Intron excision from precursor ...
Homo sapiens
Biochem. Soc. Trans.
41
831-837
2013
-
-
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1
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2
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1
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1
1
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730493
Das
Mechanism of RNA 2,3-cyclic ph ...
Escherichia virus T4
Nucleic Acids Res.
41
355-365
2013
-
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1
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1
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1
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1
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2
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737431
Ma
Simultaneous detection of kina ...
Escherichia virus T4
Anal. Biochem.
443
166-168
2013
-
-
-
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2
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1
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2
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2
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2
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1
1
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-
737681
Das
Structural and biochemical ana ...
Hungateiclostridium thermocellum
Biochemistry
52
4734-4743
2013
-
-
1
1
1
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1
-
1
-
2
-
2
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1
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8
-
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1
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1
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1
1
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1
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1
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2
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1
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8
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1
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737888
Zhang
Double strand DNA-templated co ...
Escherichia virus T4
Biosens. Bioelectron.
44
6-9
2013
-
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1
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2
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3
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Characterization of a baculovi ...
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Recognition of DNA substrates ...
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A new approach to the synthesi ...
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Mani
Spectroscopic studies of DNA a ...
Homo sapiens
Biochemistry
42
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2003
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Mutational analysis defines th ...
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Polynucleotide kinase. A versa ...
Escherichia virus T4, Mammalia
Structure
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3
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Fanta
Production, characterization, ...
Homo sapiens
Hybridoma
20
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642004
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Polynucleotide kinase (EC 2.7. ...
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Purification and kinetic prope ...
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642015
Pheiffer
Deoxyribonucleic acid kinase f ...
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Shuman
5-Hydroxyl polyribonucleotide ...
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642013
Nichols
A rapid purification of T4 pol ...
Escherichia virus T4
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Lillehaug
Inhibition of T4 polynucleotid ...
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Austin
Purification and properties of ...
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Lillehaug
Physicochemical properties of ...
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642009
Lillehaug
Phosphorylation of double-stra ...
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Biochemistry
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Levin
A deoxyribonucleic acid kinase ...
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Teraoka
Polynucleotide kinase from rat ...
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642008
Panet
Physical characterization and ...
Escherichia virus T4
Biochemistry
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5045-5050
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642005
Novogrodsky
The enzymatic phosphorylation ...
Enterobacteria phage T2, Escherichia virus T4, no activity in bacteriophage T1, no activity in bacteriophage T5
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Novogrodsky
The enzymatic phosphorylation ...
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