Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5'-dephospho-DNA | Tequatrovirus T4 | - |
ADP + 5'-phospho-DNA | - |
? | |
ATP + 5'-dephospho-DNA | Mammalia | involved in repair of chromosomal DNA strand breaks that arise continously, but at low frequency, and are potentially lethal | ADP + 5'-phospho-DNA | - |
? | |
ATP + 5'-dephospho-RNA | Tequatrovirus T4 | involved in repair of broken RNA termini | ADP + 5'-phospho-RNA | - |
? | |
additional information | Tequatrovirus T4 | - |
? | - |
? | |
additional information | Mammalia | enzyme interacts with XRCC1, a scaffold protein that helps recruit repair enzymes at sites of single-strand breakage | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mammalia | - |
- |
- |
Tequatrovirus T4 | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + 5'-dephospho-DNA = ADP + 5'-phospho-DNA | reaction mechanism | Tequatrovirus T4 | |
ATP + 5'-dephospho-DNA = ADP + 5'-phospho-DNA | structure of the active sites for 5'-polynucleotide kinase and 3'phosphatase activity of the bifunctional enzyme, substrate binding mechanism | Tequatrovirus T4 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5'-dephospho-DNA | - |
Tequatrovirus T4 | ADP + 5'-phospho-DNA | - |
? | |
ATP + 5'-dephospho-DNA | preference for recessed termini within duplex DNA | Mammalia | ADP + 5'-phospho-DNA | - |
? | |
ATP + 5'-dephospho-DNA | no preference for overhanging 5'-ends | Mammalia | ADP + 5'-phospho-DNA | - |
? | |
ATP + 5'-dephospho-DNA | preference for single stranded termini due to the narrow tunnel formation of the active site that can only bind slim molecules | Tequatrovirus T4 | ADP + 5'-phospho-DNA | - |
? | |
ATP + 5'-dephospho-DNA | involved in repair of chromosomal DNA strand breaks that arise continously, but at low frequency, and are potentially lethal | Mammalia | ADP + 5'-phospho-DNA | - |
? | |
ATP + 5'-dephospho-RNA | - |
Tequatrovirus T4 | ADP + 5'-phospho-RNA | - |
? | |
ATP + 5'-dephospho-RNA | involved in repair of broken RNA termini | Tequatrovirus T4 | ADP + 5'-phospho-RNA | - |
? | |
additional information | - |
Tequatrovirus T4 | ? | - |
? | |
additional information | poorly active on recessed termini within duplex DNA | Tequatrovirus T4 | ? | - |
? | |
additional information | enzyme interacts with XRCC1, a scaffold protein that helps recruit repair enzymes at sites of single-strand breakage | Mammalia | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Mammalia |
More | enzyme consists of 2 domains of approximately equal size, separated by a flexible loop into N-terminal half, harboring the 5'-polynucleotide kinase activity, and C-terminal half, harboring the 3'-phosphatase activity, quartenary structure | Tequatrovirus T4 |
tetramer | - |
Tequatrovirus T4 |
Synonyms | Comment | Organism |
---|---|---|
More | enzyme also possesses a 3'-phosphatase activity | Tequatrovirus T4 |