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Literature summary for 2.7.1.78 extracted from
- Purification and kinetic properties of polynucleotide kinase from rat testes (1985), Biochim. Biophys. Acta, 840, 280-286.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| spermine | 1 mM enhances activity 3times | Rattus norvegicus |  |
| spermine | inhibition above 1 mM | Rattus norvegicus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| diphosphate | - |
Rattus norvegicus | |
| phosphate | - |
Rattus norvegicus | |
| spermine | 1 mM enhances activity 3times, inhibition above 1 mM | Rattus norvegicus | |
| sulfate | - |
Rattus norvegicus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| divalent cations | absolutely required | Rattus norvegicus | |
| K+ | stimulates, optimal concentration: 0.1 M | Rattus norvegicus | |
| Mg2+ | - |
Rattus norvegicus | |
| Mn2+ | can partially replace Mg2+ in activation | Rattus norvegicus | |
| Na+ | stimulates, optimal concentration: 0.1 M | Rattus norvegicus | |
| NH4+ | stimulates, optimal concentration 0.1 M | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38000 | - |
gel filtration | Rattus norvegicus |
| 40000 | - |
1 * 40000, SDS-PAGE | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 5'-dephospho-DNA | Rattus norvegicus | - |
ADP + 5'-phospho-DNA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| over 1875fold, to homogeneity | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| testis | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.005 | - |
above, purified enzyme, 30°C | Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 10 mM Na2HPO4, pH 7.4, 50% v/v glycerol, 1 mM DTT, 0.001 mM ATP, stable for 2 months | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 5'-dephospho-DNA | - |
Rattus norvegicus | ADP + 5'-phospho-DNA | - |
? | |
| ATP + 5'-dephospho-DNA | nuclease-treated calf thymus DNA | Rattus norvegicus | ADP + 5'-phospho-DNA | - |
? | |
| ATP + 5'-dephospho-RNA | 5'-HO-tRNA is a very poor substrate | Rattus norvegicus | ADP + 5'-phospho-RNA | - |
? | |
| ATP + synthetic oligonucleotide | (dT)10 | Rattus norvegicus | ADP + oligonucleotide 5'-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 40000, SDS-PAGE | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.3 | - |
- |
Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | dependent on | Rattus norvegicus | |
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