General Stability | Organism |
---|---|
dithiothreitol protects against heat inactivation, glycerol and dithiothreitol increase stability during storage | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
adenosine | - |
Rattus norvegicus | |
deoxyadenosine | substrate ribavirin | Rattus norvegicus | |
p-chloromercuribenzoate | 0.0007 mM, 50% inhibition of ribavirin and deoxadenosine kinase activity | Rattus norvegicus | |
ribavirin | deoxyadenosine as substrate | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
deoxyadenosine | pH 7.5, 37°C | Rattus norvegicus | |
3.2 | - |
ribavirin | pH 7.5, 37°C | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Rattus norvegicus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for activity | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, Sephadex G-100, DEAE-cellulose, Sephadex G-75 | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.06 | - |
substrate ribavarin | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1-beta-D-ribofuranosyl-1,2,4-triazole-3-carboxamide | trivial name ribavirin | Rattus norvegicus | ADP + 1-beta-D-ribofuranosyl-1,2,4-triazole-3-carboxamide 5'-monophosphate | - |
? | |
ATP + adenosine | kinetic data suggest that different catalytic sites of deoxyadenosine kinase or a different enzyme are responsible for deoxyadenosine and adenosine kinase activities | Rattus norvegicus | ADP + AMP | - |
? | |
ATP + deoxyadenosine | no activity with cytidine, uridine, guanosine, deoxyguanosine and thymidine | Rattus norvegicus | ADP + dAMP | - |
? | |
ATP + ribavirin | - |
Rattus norvegicus | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
dithiothreitol protects against heat inactivation | Rattus norvegicus |
42 | - |
first-order rate constant of inactivation: in the absence of dithiothreitol, 14.2/min, in the presence of 10 mM dithiothreitol, 6.82, substrate ribavirin | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
with deoxyadenosine and ribavirin as substrates | Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | approx. 30% of maximal activity at pH 6.0 and pH 9.0, respectively | Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.035 | - |
adenosine | pH 7.5, 37°C, substrate deoxyadenosine | Rattus norvegicus | |
0.057 | - |
adenosine | pH 7.5, 37°C, substrate ribavirin | Rattus norvegicus | |
0.97 | - |
deoxyadenosine | pH 7.5, 37°C, substrate ribavirin | Rattus norvegicus | |
2.5 | - |
ribavirin | pH 7.5, 37°C, substrate deoxyadenosine | Rattus norvegicus |