Any feedback?
Literature summary for 2.7.1.76 extracted from
- The regulatory properties of deoxyadenosine kinase (1968), Biochim. Biophys. Acta, 161, 578-580.
General Stability
| General Stability | Organism |
|---|---|
| glycerol, 50%, or dithiothreitol stabilizes | Bos taurus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| dADP | 0.05 mM, 70% inhibition | Bos taurus |  |
| dAMP | 0.2 mM, 36% inhibition | Bos taurus | |
| dATP | 0.05 mM, 68% inhibition | Bos taurus | |
| dCDP | 0.001 mM, 60% inhibition | Bos taurus | |
| dCMP | 0.01 mM, 88% inhibition | Bos taurus | |
| dCTP | 0.0005 mM, 59% inhibition | Bos taurus | |
| deoxyguanosine | substrate deoxyadenosine | Bos taurus | |
| dGDP | 0.2 mM, 46% inhibition | Bos taurus | |
| dGMP | 0.5 mM, 32% inhibition | Bos taurus | |
| dGTP | 0.2 mM, 64% inhibition | Bos taurus | |
| additional information | not inhibited by adenosine | Bos taurus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5 | - |
deoxyadenosine | pH 7.8, 37°C | Bos taurus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required for activity | Bos taurus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
calf | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| streptomycin sulfate, protamine sulfate, ammonium sulfate, Sephadex G-150 | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| thymus | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
0.00006 mmol/ml min | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + deoxyadenosine | transfers a phospho group from specific nucleoside 5'-triphosphate donors to 5'-position of deoxyadenosine | Bos taurus | ADP + dAMP | - |
? | |
| ATP + deoxyadenosine | no activity with adenosine and guanosine | Bos taurus | ADP + dAMP | - |
? | |
| ATP + deoxyguanosine | - |
Bos taurus | ADP + dGMP | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | may be identical with EC 2.7.1.113, i.e. deoxyguanosine kinase | Bos taurus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Bos taurus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
