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Literature summary for 2.7.1.74 extracted from
- Gene duplications and losses among vertebrate deoxyribonucleoside kinases of the non-TK1 family (2016), Nucleosides Nucleotides Nucleic Acids, 35, 677-690 .
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00034 | - |
deoxycytidine | at pH 8.0, temperature not specified in the publication | Xenopus laevis |  |
| 0.0007 | - |
deoxycytidine | at pH 8.0, temperature not specified in the publication | Gallus gallus | |
| 0.093 | - |
deoxyadenosine | at pH 8.0, temperature not specified in the publication | Gallus gallus | |
| 0.122 | - |
deoxyguanosine | at pH 8.0, temperature not specified in the publication | Gallus gallus | |
| 0.527 | - |
deoxyguanosine | at pH 8.0, temperature not specified in the publication | Xenopus laevis | |
| 0.553 | - |
deoxyadenosine | at pH 8.0, temperature not specified in the publication | Xenopus laevis | |
| 7.502 | - |
deoxyuridine | at pH 8.0, temperature not specified in the publication | Gallus gallus | |
| 12.457 | - |
deoxythymidine | at pH 8.0, temperature not specified in the publication | Xenopus laevis | |
| 15.081 | - |
deoxyuridine | at pH 8.0, temperature not specified in the publication | Xenopus laevis | |
| 18.546 | - |
deoxythymidine | at pH 8.0, temperature not specified in the publication | Gallus gallus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Xenopus laevis | 5737 | - |
| mitochondrion | - |
Gallus gallus | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | 2.5 mM used in assay conditions | Gallus gallus | |
| Mg2+ | 2.5 mM used in assay conditions | Xenopus laevis | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + deoxycytidine | Gallus gallus | - |
ADP + dCMP | - |
? | |
| ATP + deoxycytidine | Xenopus laevis | - |
ADP + dCMP | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gallus gallus | Q5ZMF3 | - |
- |
| Xenopus laevis | A0A1L8HV70 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + deoxyadenosine | - |
Gallus gallus | ADP + dAMP | - |
? | |
| ATP + deoxyadenosine | - |
Xenopus laevis | ADP + dAMP | - |
? | |
| ATP + deoxycytidine | - |
Gallus gallus | ADP + dCMP | - |
? | |
| ATP + deoxycytidine | - |
Xenopus laevis | ADP + dCMP | - |
? | |
| ATP + deoxycytidine | highest activity | Gallus gallus | ADP + dCMP | - |
? | |
| ATP + deoxycytidine | highest activity | Xenopus laevis | ADP + dCMP | - |
? | |
| ATP + deoxyguanosine | - |
Gallus gallus | ADP + dGMP | - |
? | |
| ATP + deoxyguanosine | - |
Xenopus laevis | ADP + dGMP | - |
? | |
| ATP + deoxythymidine | lowest activity | Gallus gallus | ADP + dTMP | - |
? | |
| ATP + deoxythymidine | lowest activity | Xenopus laevis | ADP + dTMP | - |
? | |
| ATP + deoxyuridine | - |
Gallus gallus | ADP + dUMP | - |
? | |
| ATP + deoxyuridine | - |
Xenopus laevis | ADP + dUMP | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| dCK | isoform | Gallus gallus |
| dCK | isoform | Xenopus laevis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.05 | - |
deoxycytidine | at pH 8.0, temperature not specified in the publication | Gallus gallus | |
| 0.09 | - |
deoxycytidine | at pH 8.0, temperature not specified in the publication | Xenopus laevis | |
| 0.29 | - |
deoxythymidine | at pH 8.0, temperature not specified in the publication | Xenopus laevis | |
| 1.05 | - |
deoxyuridine | at pH 8.0, temperature not specified in the publication | Gallus gallus | |
| 1.08 | - |
deoxythymidine | at pH 8.0, temperature not specified in the publication | Gallus gallus | |
| 1.23 | - |
deoxyguanosine | at pH 8.0, temperature not specified in the publication | Gallus gallus | |
| 1.27 | - |
deoxyuridine | at pH 8.0, temperature not specified in the publication | Xenopus laevis | |
| 2.73 | - |
deoxyadenosine | at pH 8.0, temperature not specified in the publication | Xenopus laevis | |
| 3.4 | - |
deoxyguanosine | at pH 8.0, temperature not specified in the publication | Xenopus laevis | |
| 4.07 | - |
deoxyadenosine | at pH 8.0, temperature not specified in the publication | Gallus gallus | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0236 | - |
deoxythymidine | at pH 8.0, temperature not specified in the publication | Xenopus laevis | |
| 0.0581 | - |
deoxythymidine | at pH 8.0, temperature not specified in the publication | Gallus gallus | |
| 0.084 | - |
deoxyuridine | at pH 8.0, temperature not specified in the publication | Xenopus laevis | |
| 0.14 | - |
deoxyuridine | at pH 8.0, temperature not specified in the publication | Gallus gallus | |
| 4.94 | - |
deoxyadenosine | at pH 8.0, temperature not specified in the publication | Xenopus laevis | |
| 6.44 | - |
deoxyguanosine | at pH 8.0, temperature not specified in the publication | Xenopus laevis | |
| 10.1 | - |
deoxyguanosine | at pH 8.0, temperature not specified in the publication | Gallus gallus | |
| 43.8 | - |
deoxyadenosine | at pH 8.0, temperature not specified in the publication | Gallus gallus | |
| 65.9 | - |
deoxycytidine | at pH 8.0, temperature not specified in the publication | Gallus gallus | |
| 259 | - |
deoxycytidine | at pH 8.0, temperature not specified in the publication | Xenopus laevis | |
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