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Literature summary for 2.7.1.74 extracted from
- Potent sensitisation of cancer cells to anticancer drugs by a quadruple mutant of the human deoxycytidine kinase (2015), PLoS ONE, 10, e0140741.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21 DE3 pLysE cells | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00542 | - |
deoxycytidine | wild type enzyme, pH and temperature not specified in the publication | Homo sapiens |  |
| 0.0282 | - |
cytarabine | wild type enzyme, pH and temperature not specified in the publication | Homo sapiens | |
| 0.0467 | - |
gemcitabine | wild type enzyme, pH and temperature not specified in the publication | Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| NTP + deoxycytidine | Homo sapiens | - |
NDP + dCMP | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| His-Trap TM column chromatography and Sephacryl S-200 gel filtration | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| HEK-293T cell | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| NTP + cytarabine | - |
Homo sapiens | ? | - |
? | |
| NTP + deoxycytidine | - |
Homo sapiens | NDP + dCMP | - |
? | |
| NTP + gemcitabine | - |
Homo sapiens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | - |
Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| dCK | - |
Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.035 | - |
deoxycytidine | wild type enzyme, pH and temperature not specified in the publication | Homo sapiens | |
| 0.38 | - |
cytarabine | wild type enzyme, pH and temperature not specified in the publication | Homo sapiens | |
| 0.57 | - |
gemcitabine | wild type enzyme, pH and temperature not specified in the publication | Homo sapiens | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 6.46 | - |
deoxycytidine | wild type enzyme, pH and temperature not specified in the publication | Homo sapiens | |
| 12.21 | - |
gemcitabine | wild type enzyme, pH and temperature not specified in the publication | Homo sapiens | |
| 13.48 | - |
cytarabine | wild type enzyme, pH and temperature not specified in the publication | Homo sapiens | |
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Release 2023.1 (January 2023)
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