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Literature summary for 2.7.1.74 extracted from

  • Nomme, J.; Murphy, J.M.; Su, Y.; Sansone, N.D.; Armijo, A.L.; Olson, S.T.; Radu, C.; Lavie, A.
    Structural characterization of new deoxycytidine kinase inhibitors rationalizes the affinity-determining moieties of the molecules (2014), Acta Crystallogr. Sect. D, 70, 68-78.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 C41(DE3) cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with inhibitors and UDP, hanging drop vapor diffusion method, using 0.9-1.5 M trisodium citrate dehydrate and 25 mM HEPES pH 7.5 Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
2-[(2[-[3-(2-fluoroethoxy)-4-methoxyphenyl]-1,3-thiazol-4-yl]methyl)sulfanyl]pyrimidine-4,6-diamine
-
Homo sapiens
2-[([2-[3-(2-fluoroethoxy)-4-methoxyphenyl]-5-methyl-1,3-thiazol-4-yl]methyl)sulfanyl]pyrimidine-4,6-diamine
-
Homo sapiens
2-[([2-[3-(2-fluoroethoxy)-4-methoxyphenyl]-5-propyl-1,3-thiazol-4-yl]methyl)sulfanyl]pyrimidine-4,6-diamine
-
Homo sapiens
2-[([5-ethyl-2-[3-(2-fluoroethoxy)-4-methoxyphenyl]-1,3-thiazol-4-yl]methyl)sulfanyl]pyrimidine-4,6-diamine
-
Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Homo sapiens 5829
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + deoxycytidine Homo sapiens
-
ADP + dCMP
-
?
UTP + deoxycytidine Homo sapiens
-
UDP + dCMP
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens P27707
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap nickel-affinity column chromatography and S-200 gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + clofarabine
-
Homo sapiens ADP + clofarabine 5'-phosphate
-
?
ATP + deoxyadenosine
-
Homo sapiens ADP + dAMP
-
?
ATP + deoxycytidine
-
Homo sapiens ADP + dCMP
-
?
ATP + deoxyguanosine
-
Homo sapiens ADP + dGMP
-
?
ATP + fludarabine
-
Homo sapiens ADP + fludarabine 5'-phosphate
-
?
ATP + gemcitabine
-
Homo sapiens ADP + gemcitabine 5'-phosphate
-
?
ATP + lamivudine
-
Homo sapiens ADP + lamivudine 5'-phosphate
-
?
UTP + deoxyadenosine
-
Homo sapiens UDP + dAMP
-
?
UTP + deoxycytidine
-
Homo sapiens UDP + dCMP
-
?
UTP + deoxyguanosine
-
Homo sapiens UDP + dGMP
-
?

Subunits

Subunits Comment Organism
homodimer
-
Homo sapiens

Synonyms

Synonyms Comment Organism
dCK
-
Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0000167
-
pH and temperature not specified in the publication Homo sapiens 2-[([2-[3-(2-fluoroethoxy)-4-methoxyphenyl]-5-propyl-1,3-thiazol-4-yl]methyl)sulfanyl]pyrimidine-4,6-diamine
0.0000227
-
pH and temperature not specified in the publication Homo sapiens 2-[([5-ethyl-2-[3-(2-fluoroethoxy)-4-methoxyphenyl]-1,3-thiazol-4-yl]methyl)sulfanyl]pyrimidine-4,6-diamine
0.0000263
-
pH and temperature not specified in the publication Homo sapiens 2-[([2-[3-(2-fluoroethoxy)-4-methoxyphenyl]-5-methyl-1,3-thiazol-4-yl]methyl)sulfanyl]pyrimidine-4,6-diamine
0.0007457
-
pH and temperature not specified in the publication Homo sapiens 2-[(2[-[3-(2-fluoroethoxy)-4-methoxyphenyl]-1,3-thiazol-4-yl]methyl)sulfanyl]pyrimidine-4,6-diamine