Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 C41(DE3) cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
in complex with inhibitors and UDP, hanging drop vapor diffusion method, using 0.9-1.5 M trisodium citrate dehydrate and 25 mM HEPES pH 7.5 | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-[(2[-[3-(2-fluoroethoxy)-4-methoxyphenyl]-1,3-thiazol-4-yl]methyl)sulfanyl]pyrimidine-4,6-diamine | - |
Homo sapiens | |
2-[([2-[3-(2-fluoroethoxy)-4-methoxyphenyl]-5-methyl-1,3-thiazol-4-yl]methyl)sulfanyl]pyrimidine-4,6-diamine | - |
Homo sapiens | |
2-[([2-[3-(2-fluoroethoxy)-4-methoxyphenyl]-5-propyl-1,3-thiazol-4-yl]methyl)sulfanyl]pyrimidine-4,6-diamine | - |
Homo sapiens | |
2-[([5-ethyl-2-[3-(2-fluoroethoxy)-4-methoxyphenyl]-1,3-thiazol-4-yl]methyl)sulfanyl]pyrimidine-4,6-diamine | - |
Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + deoxycytidine | Homo sapiens | - |
ADP + dCMP | - |
? | |
UTP + deoxycytidine | Homo sapiens | - |
UDP + dCMP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P27707 | - |
- |
Purification (Comment) | Organism |
---|---|
HisTrap nickel-affinity column chromatography and S-200 gel filtration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + clofarabine | - |
Homo sapiens | ADP + clofarabine 5'-phosphate | - |
? | |
ATP + deoxyadenosine | - |
Homo sapiens | ADP + dAMP | - |
? | |
ATP + deoxycytidine | - |
Homo sapiens | ADP + dCMP | - |
? | |
ATP + deoxyguanosine | - |
Homo sapiens | ADP + dGMP | - |
? | |
ATP + fludarabine | - |
Homo sapiens | ADP + fludarabine 5'-phosphate | - |
? | |
ATP + gemcitabine | - |
Homo sapiens | ADP + gemcitabine 5'-phosphate | - |
? | |
ATP + lamivudine | - |
Homo sapiens | ADP + lamivudine 5'-phosphate | - |
? | |
UTP + deoxyadenosine | - |
Homo sapiens | UDP + dAMP | - |
? | |
UTP + deoxycytidine | - |
Homo sapiens | UDP + dCMP | - |
? | |
UTP + deoxyguanosine | - |
Homo sapiens | UDP + dGMP | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | - |
Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
dCK | - |
Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0000167 | - |
pH and temperature not specified in the publication | Homo sapiens | 2-[([2-[3-(2-fluoroethoxy)-4-methoxyphenyl]-5-propyl-1,3-thiazol-4-yl]methyl)sulfanyl]pyrimidine-4,6-diamine | |
0.0000227 | - |
pH and temperature not specified in the publication | Homo sapiens | 2-[([5-ethyl-2-[3-(2-fluoroethoxy)-4-methoxyphenyl]-1,3-thiazol-4-yl]methyl)sulfanyl]pyrimidine-4,6-diamine | |
0.0000263 | - |
pH and temperature not specified in the publication | Homo sapiens | 2-[([2-[3-(2-fluoroethoxy)-4-methoxyphenyl]-5-methyl-1,3-thiazol-4-yl]methyl)sulfanyl]pyrimidine-4,6-diamine | |
0.0007457 | - |
pH and temperature not specified in the publication | Homo sapiens | 2-[(2[-[3-(2-fluoroethoxy)-4-methoxyphenyl]-1,3-thiazol-4-yl]methyl)sulfanyl]pyrimidine-4,6-diamine |