Crystallization (Comment) | Organism |
---|---|
dCK in complex with acyclovir at the nucleoside phosphoryl acceptor site, and UDP at the phosphoryl donor site, hanging drop vapour diffusion method, 20 mg/ml protein with 5 mM UDP and 5 mM acyclovir is mixed with reservoir solution containing 0.90-1.5 M trisodium citrate dihydrate and 100 mM HEPES, pH 7.5, 12°C, X-ray diffraction structure determination and analysis at 2.1 A resolution, molecular replacement | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + deoxyadenosine | Homo sapiens | - |
ADP + dAMP | - |
? | |
ATP + deoxycytidine | Homo sapiens | - |
ADP + dCMP | - |
? | |
ATP + deoxyguanosine | Homo sapiens | - |
ADP + dGMP | - |
? | |
UTP + deoxyadenosine | Homo sapiens | - |
UDP + dAMP | - |
? | |
UTP + deoxycytidine | Homo sapiens | - |
UDP + dCMP | - |
? | |
UTP + deoxyguanosine | Homo sapiens | - |
UDP + dGMP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P27707 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + deoxyadenosine | - |
Homo sapiens | ADP + dAMP | - |
? | |
ATP + deoxycytidine | - |
Homo sapiens | ADP + dCMP | - |
? | |
ATP + deoxyguanosine | - |
Homo sapiens | ADP + dGMP | - |
? | |
additional information | the human enzyme is not active with the acyclic guanine analogue acyclovir. Acyclovir binds at the dCK active site, but does so adopting a nonproductive conformation. Despite binding ACV, the enzyme remains in the open, inactive state, overview. dCK would phosphorylate acyclic guanine analogs if they can induce a similar rotation | Homo sapiens | ? | - |
? | |
UTP + deoxyadenosine | - |
Homo sapiens | UDP + dAMP | - |
? | |
UTP + deoxycytidine | - |
Homo sapiens | UDP + dCMP | - |
? | |
UTP + deoxyguanosine | - |
Homo sapiens | UDP + dGMP | - |
? |
Synonyms | Comment | Organism |
---|---|---|
dCK | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens | |
UTP | - |
Homo sapiens |