Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli and in HEK-293 cell | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
S74A | 6-8fold lower activity than wild-type | Homo sapiens |
S74D | 2-4fold lower kcat values | Homo sapiens |
S74E | mutation mimicking phosphorylation, results in 11fold increase in kcat value for deoxycytidine. Mutation has no effect on cellular localization | Homo sapiens |
S74Q | no significant change in kcat for any of the substrates tested | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0017 | - |
2'-deoxycytidine | wild-type, pH 7.5, 25°C | Homo sapiens | |
0.0059 | - |
1-beta-D-arabinofuranosylcytosine | wild-type, pH 7.5, 25°C | Homo sapiens | |
0.0062 | - |
2',2'-difluorodeoxycytidine | wild-type, pH 7.5, 25°C | Homo sapiens | |
0.0089 | - |
2'-deoxycytidine | mutant S74E, pH 7.5, 25°C | Homo sapiens | |
0.015 | - |
2',2'-difluorodeoxycytidine | mutant S74E, pH 7.5, 25°C | Homo sapiens | |
0.024 | - |
1-beta-D-arabinofuranosylcytosine | mutant S74E, pH 7.5, 25°C | Homo sapiens | |
0.1169 | - |
2'-deoxyadenosine | wild-type, pH 7.5, 25°C | Homo sapiens | |
0.2458 | - |
2'-deoxyguanosine | wild-type, pH 7.5, 25°C | Homo sapiens | |
0.518 | - |
2'-deoxyadenosine | mutant S74E, pH 7.5, 25°C | Homo sapiens | |
0.602 | - |
2'-deoxyguanosine | mutant S74E, pH 7.5, 25°C | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | main localization of both wild-type and mutant S74E mimicking phosphorylated enzyme | Homo sapiens | 5634 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
x * 30000, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P27707 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | phosphorylation at residue S74 | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1-beta-D-arabinofuranosylcytosine | - |
Homo sapiens | ADP + 1-beta-D-arabinofuranosylcytosine 5'-phosphate | - |
? | |
ATP + 2',2'-difluorodeoxycytidine | - |
Homo sapiens | ADP + 2',2'-difluorodeoxycytidine 5'-phosphate | - |
? | |
ATP + 2'-deoxyadenosine | - |
Homo sapiens | ADP + 2'-deoxyadenosine 5'-phosphate | - |
? | |
ATP + 2'-deoxycytidine | - |
Homo sapiens | ADP + 2'-deoxy-CMP | - |
? | |
ATP + 2'-deoxyguanosine | - |
Homo sapiens | ADP + 2'-deoxyguanosine 5'-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 30000, SDS-PAGE | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
2'-deoxycytidine | wild-type, pH 7.5, 25°C | Homo sapiens | |
0.04 | - |
2'-deoxycytidine | mutant S74A, pH 7.5, 25°C | Homo sapiens | |
0.04 | - |
2'-deoxycytidine | mutant S74Q, pH 7.5, 25°C | Homo sapiens | |
0.16 | - |
2'-deoxycytidine | mutant S74D, pH 7.5, 25°C | Homo sapiens | |
0.3 | - |
1-beta-D-arabinofuranosylcytosine | mutant S74Q, pH 7.5, 25°C | Homo sapiens | |
0.32 | - |
1-beta-D-arabinofuranosylcytosine | wild-type, pH 7.5, 25°C | Homo sapiens | |
0.33 | - |
1-beta-D-arabinofuranosylcytosine | mutant S74A, pH 7.5, 25°C | Homo sapiens | |
0.36 | - |
2'-deoxyguanosine | mutant S74E, pH 7.5, 25°C | Homo sapiens | |
0.44 | - |
2',2'-difluorodeoxycytidine | wild-type, pH 7.5, 25°C | Homo sapiens | |
0.45 | - |
2'-deoxycytidine | mutant S74E, pH 7.5, 25°C | Homo sapiens | |
0.45 | - |
2',2'-difluorodeoxycytidine | mutant S74Q, pH 7.5, 25°C | Homo sapiens | |
0.5 | - |
2',2'-difluorodeoxycytidine | mutant S74A, pH 7.5, 25°C | Homo sapiens | |
0.57 | - |
2'-deoxyguanosine | mutant S74D, pH 7.5, 25°C | Homo sapiens | |
0.57 | - |
1-beta-D-arabinofuranosylcytosine | mutant S74D, pH 7.5, 25°C | Homo sapiens | |
0.65 | - |
2'-deoxyguanosine | mutant S74Q, pH 7.5, 25°C | Homo sapiens | |
0.68 | - |
2'-deoxyguanosine | mutant S74A, pH 7.5, 25°C | Homo sapiens | |
0.69 | - |
2'-deoxyadenosine | mutant S74E, pH 7.5, 25°C | Homo sapiens | |
0.73 | - |
2'-deoxyguanosine | wild-type, pH 7.5, 25°C | Homo sapiens | |
0.77 | - |
2'-deoxyadenosine | wild-type, pH 7.5, 25°C | Homo sapiens | |
0.9 | - |
2',2'-difluorodeoxycytidine | mutant S74D, pH 7.5, 25°C | Homo sapiens | |
0.9 | - |
2'-deoxyadenosine | mutant S74Q, pH 7.5, 25°C | Homo sapiens | |
1 | - |
2'-deoxyadenosine | mutant S74D, pH 7.5, 25°C | Homo sapiens | |
1.06 | - |
1-beta-D-arabinofuranosylcytosine | mutant S74E, pH 7.5, 25°C | Homo sapiens | |
1.12 | - |
2'-deoxyadenosine | mutant S74A, pH 7.5, 25°C | Homo sapiens | |
1.21 | - |
2',2'-difluorodeoxycytidine | mutant S74E, pH 7.5, 25°C | Homo sapiens |