Application | Comment | Organism |
---|---|---|
medicine | sequencing of gene from European and African individuals reveals 64 genetic polymorphisms. In general, African ancestry subjects show higher mRNA expression compared with subjects with European ancestry. In both groups, single nucleotide polymorphism 35708 C>T of a 3'-untranslated region is significantly associated with lower mRNA expression and with lower blast 1-beta-D-arabinofuranosylcytosine 5'-triphosphate levels in acute myeloid leukemia patients | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
native and mutant proteins expressed in Escherichia coli BL21 star | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
A119G | naturally occurring mutation, 66% of activity of the wild type protein | Homo sapiens |
A119G | genetic polymorphism, 66% of wild-type activity, decrease in Km value | Homo sapiens |
I24V | naturally occurring mutation, 85% of activity of the wild type protein | Homo sapiens |
I24V | genetic polymorphism, 85% of wild-type activity | Homo sapiens |
additional information | sequencing of gene from European and African individuals reveals 64 genetic polymorphisms. In general, African ancestry subjects show higher mRNA expression compared with subjects with European ancestry. In both groups, single nucleotide polymorphism 35708 C>T of a 3'-untranslated region is significanlty associated with lower mRNA expression and with lower blast 1-beta-D-arabinofuranosylcytosine 5'-triphosphate levels in acute myeloid leukemia patients | Homo sapiens |
P122S | naturally occurring mutation, 43% of activity of the wild type protein | Homo sapiens |
P122S | genetic polymorphism, 43% of wild-type activity, decrease in Km value | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
2-chloro-2'-deoxyadenosine | A119G mutant enzyme, 37°C, pH 7.4 | Homo sapiens | |
0.004 | - |
2-chloro-2'-deoxyadenosine | P122S mutant enzyme, 37°C, pH 7.4 | Homo sapiens | |
0.006 | - |
2-chloro-2'-deoxyadenosine | wild type enzyme, 37°C, pH 7.4 | Homo sapiens | |
0.009 | - |
2-chloro-2'-deoxyadenosine | I24V mutant enzyme, 37°C, pH 7.4 | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | in complex with ATP | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
NTP + 1-beta-D-arabinofuranosylcytosine | Homo sapiens | i.e. cytarabine, rate limiting enzyme in the activation of the prodrug | NDP + 1-beta-D-arabinofuranosylcytosine 5'-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
differences in mRNA levels in African and European individuals detected | - |
Homo sapiens | P27707 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
B-lymphoblastoid cell line | - |
Homo sapiens | - |
lymphoblast | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2-chloro-2'-deoxyadenosine | i.e. cladribine | Homo sapiens | ADP + 2-chloro-2'-deoxyadenosine 5'-phosphate | - |
? | |
NTP + 1-beta-D-arabinofuranosylcytosine | i.e. cytarabine, rate limiting enzyme in the activation of the prodrug | Homo sapiens | NDP + 1-beta-D-arabinofuranosylcytosine 5'-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
dCK | - |
Homo sapiens |