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Literature summary for 2.7.1.74 extracted from

  • Eriksson, S.; Munch-Petersen, B.; Johansson, K.; Eklund, H.
    Structure and function of cellular deoxyribonucleoside kinases (2002), Cell. Mol. Life Sci., 59, 1327-1346.
    View publication on PubMed
Search for more literature for 2.7.1.74

Cloned(Commentary)

Cloned (Comment) Organism
the single copy gene is located on chromosome 4q13.3-q21.1 Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
dCTP feedback inhibition, competitive versus phosphate donors Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information no Michaelis-Menten kinetics, but negative cooperativity Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Homo sapiens 5737
-
nucleus enzyme possesses a nuclear localization signal at the N-terminus Homo sapiens 5634
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 2'-deoxycytidine Homo sapiens
-
 ADP + 2'-deoxycytidine 5'-phosphate
-
 ?
UTP + 2'-deoxycytidine Homo sapiens
-
 UDP + 2'-deoxycytidine 5'-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P27707
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information posttranscriptional regulation of the enzyme Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
NTP + deoxycytidine = NDP + dCMP substrate-enzyme interactions, bi bi random reaction mechanism with ATP, but ordered with UTP binding before the acceptor substrate, substrate binding site structures Homo sapiens
a

Source Tissue

Source Tissue Comment Organism Textmining
colonic mucosa
-
 Homo sapiens
-
kidney very low enzyme expression Homo sapiens
-
liver very low enzyme expression Homo sapiens
-
lymphoid tissue high enzyme expression Homo sapiens
-
additional information tissue specific enzyme expression Homo sapiens
-
muscle very low enzyme expression Homo sapiens
-
pancreas very low enzyme expression Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 substrate specificity Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2',3'-dideoxycytidine i.e. zalcitabine Homo sapiens ADP + 2',3'-dideoxycytidine 5'-phosphate
-
 ?
ATP + 2'-deoxyadenosine
-
 Homo sapiens ADP + 2'-deoxyadenosine 5'-phosphate
-
 ?
ATP + 2'-deoxycytidine
-
 Homo sapiens ADP + 2'-deoxycytidine 5'-phosphate
-
 ?
ATP + 2'-deoxyguanosine
-
 Homo sapiens ADP + 2'-deoxyguanosine 5'-phosphate
-
 ?
ATP + 2-chloro-2'-deoxyadenosine i.e. cladribine Homo sapiens ADP + 2-chloro-2'-deoxyadenosine 5'-phosphate
-
 ?
ATP + arabinosyl adenine
-
 Homo sapiens ADP + arabinosyl adenine 5'-phosphate
-
 ?
ATP + arabinosyl cytosine i.e. cytosar Homo sapiens ADP + arabinosyl cytosine 5'-phosphate
-
 ?
ATP + beta-L-2',3'-dideoxy-3'-thiacytidine
-
 Homo sapiens ADP + beta-L-2',3'-dideoxy-3'-thiacytidine 5'-phosphate
-
 ?
ATP + difluoro-arabinofuranosyl adenine i.e. fludarabine Homo sapiens ADP + difluoro-arabinofuranosyl adenine 5'-phosphate
-
 ?
ATP + difluorodeoxycytidine i.e. gemcitabine Homo sapiens ADP + difluorodeoxycytidine 5'-phosphate
-
 ?
additional information substrate-enzyme interactions, overview, enzyme prefers nucleosides with sugars in the S-conformation, i.e C2'-endo-C3'-exo, binding of substrate induces conformational changes, the phosphate donor influences the acceptor substrate specificity, mechanism, overview Homo sapiens ?
-
 ?
UTP + 2',3'-dideoxycytidine i.e. zalcitabine Homo sapiens UDP + 2',3'-dideoxycytidine 5'-phosphate
-
 ?
UTP + 2'-deoxyadenosine
-
 Homo sapiens UDP + 2'-deoxyadenosine 5'-phosphate
-
 ?
UTP + 2'-deoxycytidine
-
 Homo sapiens UDP + 2'-deoxycytidine 5'-phosphate
-
 ?
UTP + 2'-deoxyguanosine
-
 Homo sapiens UDP + 2'-deoxyguanosine 5'-phosphate
-
 ?
UTP + 2-chloro-2'-deoxyadenosine i.e. cladribine Homo sapiens UDP + 2-chloro-2'-deoxyadenosine 5'-phosphate
-
 ?
UTP + arabinosyl adenine
-
 Homo sapiens UDP + arabinosyl adenine 5'-phosphate
-
 ?
UTP + arabinosyl cytosine i.e. cytosar Homo sapiens UDP + arabinosyl cytosine 5'-phosphate
-
 ?
UTP + beta-L-2',3'-dideoxy-3'-thiacytidine
-
 Homo sapiens UDP + beta-L-2',3'-dideoxy-3'-thiacytidine 5'-phosphate
-
 ?
UTP + beta-L-dioxolane-cytidine antitumor drug Homo sapiens UDP + beta-L-dioxolane-cytidine 5'-phosphate
-
 ?
UTP + difluoro-arabinofuranosyl adenine i.e. fludarabine Homo sapiens UDP + difluoro-arabinofuranosyl adenine 5'-phosphate
-
 ?
UTP + difluorodeoxycytidine i.e. gemcitabine Homo sapiens UDP + difluorodeoxycytidine 5'-phosphate
-
 ?

Subunits

Subunits Comment Organism
More 3D-structure analysis, dCK structure model, overview Homo sapiens

Synonyms

Synonyms Comment Organism
dCK
-
 Homo sapiens
NTP:deoxycytidine 5'-phosphotransferase
-
 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Homo sapiens
additional information enzyme lalso utilizes other nucleoside triphosphates as phosphate donors Homo sapiens
UTP
-
 Homo sapiens