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Literature summary for 2.7.1.74 extracted from

  • Kim, M.Y.; Ikeda, S.; Ives, D.H.
    Affinity purification of human deoxycytidine kinase: avoidance of structural and kinetic artifacts arising from limited proteolysis (1988), Biochem. Biophys. Res. Commun., 156, 92-98.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0033 0.0093 deoxycytidine
-
Homo sapiens
0.64
-
deoxyguanosine 37°C, pH 7.5 Homo sapiens
0.89
-
deoxyadenosine 37°C, pH 7.5 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59300
-
sedimentation equilibrium determination, PAGE, both in the presence of protease inhibitors Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
affinity chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
leukemia cell leukemic T-lymphoblasts Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2'-deoxycytidine
-
Homo sapiens ADP + 5'-dCMP
-
?
CTP + 2'-deoxycytidine
-
Homo sapiens CDP + 2'-deoxy-CMP
-
?
dGTP + 2'-deoxycytidine
-
Homo sapiens dGDP + 2'-deoxy-CMP
-
?
dTTP + 2'-deoxycytidine
-
Homo sapiens dTDP + 2'-deoxy-CMP
-
?
GTP + 2'-deoxycytidine
-
Homo sapiens GDP + 2'-deoxy-CMP
-
?
additional information purine deoxynucleoside activity inseparably associated with deoxycytidine kinase protein Homo sapiens ?
-
?
NTP + deoxyadenosine
-
Homo sapiens NDP + dAMP
-
?
NTP + deoxycytidine no substrate: dCTP Homo sapiens NDP + dCMP
-
?
NTP + deoxyguanosine
-
Homo sapiens NDP + dGMP
-
?
UTP + 2'-deoxycytidine
-
Homo sapiens UDP + 2'-deoxy-CMP
-
?

Subunits

Subunits Comment Organism
monomer
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens