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Literature summary for 2.7.1.73 extracted from

  • Lee, W.H.; Shin, S.Y.; Kim, M.D.; Han, N.S.; Seo, J.H.
    Modulation of guanosine nucleotides biosynthetic pathways enhanced GDP-L-fucose production in recombinant Escherichia coli (2012), Appl. Microbiol. Biotechnol., 93, 2327-2334.
    View publication on PubMed
Search for more literature for 2.7.1.73

Cloned(Commentary)

Cloned (Comment) Organism
gene gsk, several metabolic enzymes such as GMP synthetase and IMP dehydrogenase, GMP reductase, and guanosine-inosine kinase, all involved in the biosynthesis of GMP, are co-overexpressed in recombinant Escherichia coli TOP10 and BL21star(DE3) cells becoming able to produce GDP-L-fucose, pH-stat fed-batch fermentations quantification and optimization, overview Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information overexpression of Gsk in the fed-batch fermentation in recombinant Escherichia coli strain BL21star(DE3) with glucose as the sole carbon source, leads to a significant improvement of GDP-L-fucose production, profiles of cell growth and GDP-L-fucose production, overview Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + guanosine Escherichia coli
-
 ADP + GMP
-
 ?
ATP + inosine Escherichia coli
-
 ADP + IMP
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 ATCC 10798, gene gsk
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + guanosine
-
 Escherichia coli ADP + GMP
-
 ?
ATP + inosine
-
 Escherichia coli ADP + IMP
-
 ?

Synonyms

Synonyms Comment Organism
Gsk
-
 Escherichia coli
guanosine-inosine kinase
-
 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Escherichia coli

General Information

General Information Comment Organism
metabolism in the salvage pathway, guanosine and inosine are directly phosphorylated to GMP and IMP, respectively, by guanosine-inosine kinase, or guanosine is indirectly converted to GMP through serial phosphorolysis and phosphorylation catalyzed by purine nucleoside phosphorylase and guanine phosphoribosyltransferase, respectively, pathway, overview Escherichia coli