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Literature summary for 2.7.1.73 extracted from

  • Mori, H.; Iida, A.; Teshiba, S.; Fujio, T.
    Cloning of a guanosine-inosine kinase gene of Escherichia coli and characterization of the purified gene product (1995), J. Bacteriol., 177, 4921-4926.
    View publication on PubMed
Search for more literature for 2.7.1.73

Activating Compound

Activating Compound Comment Organism Structure
K+ the enzyme requires both Mg2+ and K+ Escherichia coli
Mg2+ enzyme requires both Mg2+ and K+ Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
-
 Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
 Escherichia coli
Zn2+
-
 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0061
-
 guanosine pH 7.2, 30°C Escherichia coli
0.51
-
 ATP pH 7.2, 30°C, guanosine kinase reaction Escherichia coli
0.66
-
 dATP pH 7.2, 30°C, inosine kinase reaction Escherichia coli
0.71
-
 ATP pH 7.2, 30°C, inosine kinase reaction Escherichia coli
2.1
-
 Inosine pH 7.2, 30°C Escherichia coli
2.4
-
 dATP pH 7.2, 30°C, guanosine kinase reaction Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48400
-
 x * 48400, calculation from nucleotide sequence Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P0AEW6
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.7
-
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + guanosine
-
 Escherichia coli ADP + GMP
-
 ?
ATP + inosine about 60% of the activity with guanosine Escherichia coli ADP + 5'-IMP
-
 ?
dATP + guanosine
-
 Escherichia coli dADP + GMP
-
 ?
dATP + inosine
-
 Escherichia coli dADP + IMP
-
 ?
UTP + guanosine UTP shows 20% of the activity with ATP Escherichia coli UDP + GMP
-
 ?
UTP + inosine UTP shows 20% of the activity with ATP Escherichia coli UDP + IMP
-
 ?

Subunits

Subunits Comment Organism
? x * 48400, calculation from nucleotide sequence Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
26 39 inosine kinase Escherichia coli
38
-
 guanosine kinase Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.9
-
 inosine kinase Escherichia coli
8.2
-
 guanosine kinase Escherichia coli