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Literature summary for 2.7.1.73 extracted from
- Cloning of a guanosine-inosine kinase gene of Escherichia coli and characterization of the purified gene product (1995), J. Bacteriol., 177, 4921-4926.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| K+ | the enzyme requires both Mg2+ and K+ | Escherichia coli |  |
| Mg2+ | enzyme requires both Mg2+ and K+ | Escherichia coli | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Escherichia coli | |
| Zn2+ | - |
Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0061 | - |
guanosine | pH 7.2, 30°C | Escherichia coli | |
| 0.51 | - |
ATP | pH 7.2, 30°C, guanosine kinase reaction | Escherichia coli | |
| 0.66 | - |
dATP | pH 7.2, 30°C, inosine kinase reaction | Escherichia coli | |
| 0.71 | - |
ATP | pH 7.2, 30°C, inosine kinase reaction | Escherichia coli | |
| 2.1 | - |
Inosine | pH 7.2, 30°C | Escherichia coli | |
| 2.4 | - |
dATP | pH 7.2, 30°C, guanosine kinase reaction | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48400 | - |
x * 48400, calculation from nucleotide sequence | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P0AEW6 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.7 | - |
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + guanosine | - |
Escherichia coli | ADP + GMP | - |
? | |
| ATP + inosine | about 60% of the activity with guanosine | Escherichia coli | ADP + 5'-IMP | - |
? | |
| dATP + guanosine | - |
Escherichia coli | dADP + GMP | - |
? | |
| dATP + inosine | - |
Escherichia coli | dADP + IMP | - |
? | |
| UTP + guanosine | UTP shows 20% of the activity with ATP | Escherichia coli | UDP + GMP | - |
? | |
| UTP + inosine | UTP shows 20% of the activity with ATP | Escherichia coli | UDP + IMP | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 48400, calculation from nucleotide sequence | Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 26 | 39 | inosine kinase | Escherichia coli |
| 38 | - |
guanosine kinase | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.9 | - |
inosine kinase | Escherichia coli |
| 8.2 | - |
guanosine kinase | Escherichia coli |
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