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Literature summary for 2.7.1.72 extracted from
- Purification and characterization of aminoglycoside phosphotransferase APH(6)-Id, a streptomycin-inactivating enzyme (2014), Mol. Cell. Biochem., 387, 207-216.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli Rosetta(DE3)pLysS cells | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.38 | - |
streptomycin | at pH 7.5 and 25°C | Escherichia coli |  |
| 1.03 | - |
ATP | at pH 7.5 and 25°C | Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
1 * 30000, SDS-PAGE | Escherichia coli |
| 31400 | - |
gel filtration | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + streptomycin | Escherichia coli | - |
ADP + streptomycin 6-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + streptomycin | - |
Escherichia coli | ADP + streptomycin 6-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 30000, SDS-PAGE | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| aminoglycoside phosphotransferase | - |
Escherichia coli |
| APH(6)-Id | - |
Escherichia coli |
| streptomycin phosphotransferase | - |
Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.7 | - |
ATP | at pH 7.5 and 25°C | Escherichia coli | |
| 1.7 | - |
streptomycin | at pH 7.5 and 25°C | Escherichia coli | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the enzyme confers resistance to streptomycin | Escherichia coli |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.5 | - |
streptomycin | at pH 7.5 and 25°C | Escherichia coli | |
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Release 2023.1 (January 2023)
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