Literature summary for 2.7.1.71 extracted from

Nugroho, W.S.; Kim, D.W.; Han, J.C.; Hur, Y.B.; Nam, S.W.; Kim, H.J.
Cloning, expression, and characterization of a cold-adapted shikimate kinase from the psychrophilic bacterium Colwellia psychrerythraea 34H (2016), J. Microbiol. Biotechnol., 26, 2087-2097 .

Search for more literature for 2.7.1.71

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Colwellia psychrerythraea
expressed in Escherichia coli BL21(DE3) cells	Escherichia coli

General Stability

General Stability	Organism
the urea concentration required for half-maximal denaturation is 1.5 mM	Colwellia psychrerythraea
the urea concentration required for half-maximal denaturation is 2.5 mM	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.1	-	shikimate	at pH 7.5 and 25°C	Colwellia psychrerythraea
0.153	-	ATP	at pH 7.5 and 25°C	Colwellia psychrerythraea
0.362	-	shikimate	at pH 7.5 and 25°C	Escherichia coli
0.5	-	ATP	at pH 7.5 and 25°C	Escherichia coli

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	15 mM used in assay conditions	Colwellia psychrerythraea
Mg2+	15 mM used in assay conditions	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + shikimate	Colwellia psychrerythraea	-	ADP + 3-phosphoshikimate	-	r
ATP + shikimate	Escherichia coli	-	ADP + 3-phosphoshikimate	-	r

Organism

Organism	UniProt	Comment	Textmining
Colwellia psychrerythraea	-	-	-
Escherichia coli	P0A6D7	-	-

Purification (Commentary)

Purification (Comment)	Organism
His-Bind agarose resin column chromatography	Colwellia psychrerythraea
His-Bind agarose resin column chromatography	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + shikimate	-	Colwellia psychrerythraea	ADP + 3-phosphoshikimate	-	r
ATP + shikimate	-	Escherichia coli	ADP + 3-phosphoshikimate	-	r

Subunits

Subunits	Comment	Organism
?	x * about 20000, SDS-PAGE	Colwellia psychrerythraea
?	x * about 20000, SDS-PAGE	Escherichia coli
?	x * 19490, calculated from amino acid sequence	Colwellia psychrerythraea

Synonyms

Synonyms	Comment	Organism
AroK	-	Colwellia psychrerythraea
AroK	-	Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	-	Colwellia psychrerythraea
40	-	-	Escherichia coli

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
10	40	the enzyme retains about 40% of its maximal activity at 10°C and 40°C	Colwellia psychrerythraea
15	40	the enzyme retains about 40% of its maximal activity at 15°C and 40°C	Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
33	-	melting temperature	Colwellia psychrerythraea
45	-	melting temperature	Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
28.8	-	shikimate	at pH 7.5 and 25°C	Colwellia psychrerythraea
32.8	-	ATP	at pH 7.5 and 25°C	Colwellia psychrerythraea
55.86	-	shikimate	at pH 7.5 and 25°C	Escherichia coli
122.51	-	ATP	at pH 7.5 and 25°C	Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
154	-	shikimate	at pH 7.5 and 25°C	Escherichia coli
214	-	ATP	at pH 7.5 and 25°C	Colwellia psychrerythraea
245	-	ATP	at pH 7.5 and 25°C	Escherichia coli
288	-	shikimate	at pH 7.5 and 25°C	Colwellia psychrerythraea

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Release 2023.1 (January 2023)