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Literature summary for 2.7.1.71 extracted from

  • Sutton, K.A.; Breen, J.; MacDonald, U.; Beanan, J.M.; Olson, R.; Russo, T.A.; Schultz, L.W.; Umland, T.C.
    Structure of shikimate kinase, an in vivo essential metabolic enzyme in the nosocomial pathogen Acinetobacter baumannii, in complex with shikimate (2015), Acta Crystallogr. Sect. D, 71, 1736-1744.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta (DE3) cells Acinetobacter baumannii

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with shikimate, microbatch-under-oil method and sitting-drop vapor diffusion method using 0.1 M HEPES pH 7.5, 1.5 M lithium sulfate Acinetobacter baumannii

Inhibitors

Inhibitors Comment Organism Structure
shikimate substrate inhibition Acinetobacter baumannii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.147
-
shikimate at pH 7.5 and 25°C Acinetobacter baumannii

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Acinetobacter baumannii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21500
-
gel filtration Acinetobacter baumannii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + shikimate Acinetobacter baumannii
-
ADP + 3-phosphoshikimate
-
?
ATP + shikimate Acinetobacter baumannii 307-0294
-
ADP + 3-phosphoshikimate
-
?

Organism

Organism UniProt Comment Textmining
Acinetobacter baumannii A0A0M3KL09
-
-
Acinetobacter baumannii 307-0294 A0A0M3KL09
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography, and Superdex 75 gel filtration Acinetobacter baumannii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + shikimate
-
Acinetobacter baumannii ADP + 3-phosphoshikimate
-
?
ATP + shikimate
-
Acinetobacter baumannii 307-0294 ADP + 3-phosphoshikimate
-
?

Subunits

Subunits Comment Organism
monomer 1 * 21500, SDS-PAGE Acinetobacter baumannii

Synonyms

Synonyms Comment Organism
AroK
-
Acinetobacter baumannii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
21.4
-
shikimate at pH 7.5 and 25°C Acinetobacter baumannii

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.95
-
shikimate at pH 7.5 and 25°C Acinetobacter baumannii