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Literature summary for 2.7.1.71 extracted from

  • de Feyter, R.
    Shikimate kinases from Escherichia coli K12 (1987), Methods Enzymol., 142, 355-361.
    View publication on PubMed
Search for more literature for 2.7.1.71

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.16
-
 ATP isoenzyme SK2, at 1 mM shikimate Escherichia coli
0.2
-
 shikimate isoenzyme SK2, at 5 mM ATP Escherichia coli
5
-
 shikimate above, isoenzyme SK1, at 5 mM Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ divalent cations required, Mg2+ is most effective, but significant activity is obtained with Fe2+, Ca2+, Mn2+ or Co2+, isoenzyme SK2 Escherichia coli
Co2+ divalent cations required, Mg2+ is most effective, but significant activity is obtained with Fe2+, Ca2+, Mn2+ or Co2+, isoenzyme SK2 Escherichia coli
Fe2+ divalent cations required, Mg2+ is most effective, but significant activity is obtained with Fe2+, Ca2+, Mn2+ or Co2+, isoenzyme SK2 Escherichia coli
Mg2+ divalent cations required, Mg2+ is most effective, but significant activity is obtained with Fe2+, Ca2+, Mn2+ or Co2+, isoenzyme SK2 Escherichia coli
Mn2+ divalent cations required, Mg2+ is most effective, but significant activity is obtained with Fe2+, Ca2+, Mn2+ or Co2+, isoenzyme SK2 Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
19000
-
 isoenzyme SK1, gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + shikimate Escherichia coli SK2 is the isoenzyme that normally functions in aromatic biosynthesis in the cell, SK1 functions only when high intracellular levels of shikimate occurs ADP + shikimate 3-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli
-
 two isoenzymes: SK1 and SK2
-

Purification (Commentary)

Purification (Comment) Organism
isoenzyme SK1 and SK2 Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
100
-
 isoenzyme SK2 Escherichia coli
410
-
 isoenzyme SK1 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + shikimate
-
 Escherichia coli ADP + shikimate 3-phosphate
-
 ?
ATP + shikimate SK2 is the isoenzyme that normally functions in aromatic biosynthesis in the cell, SK1 functions only when high intracellular levels of shikimate occurs Escherichia coli ADP + shikimate 3-phosphate
-
 ?