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Literature summary for 2.7.1.7 extracted from

  • Sebastian, J.; Asensio, C.
    Purification and properties of the mannokinase from Escherichia coli (1972), Arch. Biochem. Biophys., 151, 227-233.
    View publication on PubMed
Search for more literature for 2.7.1.7

Inhibitors

Inhibitors Comment Organism Structure
D-fructose competitive to glucose Escherichia coli
D-mannosamine competitive Escherichia coli
D-mannose competitive to glucose Escherichia coli
D-mannose 6-phosphate up to 20 mM no effect Escherichia coli
L-sorbose competitive Escherichia coli
lyxose competitive Escherichia coli
MgADP-
-
 Escherichia coli
Mn2+ at a Mn2+/ATP ratio of more than 2 Escherichia coli
additional information up to 20 mM fructose 6-phosphate shows no inhibitory effect Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
 ATP reaction with D-mannose Escherichia coli
0.2
-
 ATP ATP in form of MgATP2- Escherichia coli
0.3
-
 D-fructose pH 7.5, 25°C Escherichia coli
0.3
-
 ITP pH 7.5, 25°C Escherichia coli
0.5
-
 GTP pH 7.5, 25°C Escherichia coli
0.6
-
 dATP pH 7.5, 25°C Escherichia coli
2
-
 CTP pH 7.5, 25°C Escherichia coli
2
-
 UTP pH 7.5, 25°C Escherichia coli
4
-
 D-glucose pH 7.5, 25°C Escherichia coli
4
-
 TTP pH 7.5, 25°C Escherichia coli
5
-
 D-glucosamine pH 7.5, 25°C Escherichia coli
10
-
 D-glucose pH 7.5, 25°C Escherichia coli
10
-
 2-deoxyglucose pH 7.5, 25°C Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ can partially replace Mg2+ Escherichia coli
Mg2+ required Escherichia coli
Mn2+ can partially replace Mg2+ Escherichia coli
additional information no activation with Ca2+, Sn2+, Cd2+ Escherichia coli
Ni2+ 40% of the activity with Mg2+ Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5
-
-
 Escherichia coli

Storage Stability

Storage Stability Organism
-10°C, stable for several months Escherichia coli
4°C, stable for several days Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2-deoxy-D-glucose 20% of the activity with D-mannose Escherichia coli ADP + 2-deoxy-D-glucose 6-phosphate
-
 ?
ATP + 2-deoxy-D-mannose no activity Escherichia coli ADP + 2-deoxy-D-mannose 6-phosphate
-
 ?
ATP + D-fructose D-mannose and D-fructose phosphorylated equally well Escherichia coli ADP + D-fructose 6-phosphate
-
 ?
ATP + D-glucosamine 5% of the activity with D-mannose Escherichia coli ADP + D-glucosamine 6-phosphate
-
 ?
ATP + D-glucose 50% of the activity with D-mannose Escherichia coli ADP + D-glucose 6-phosphate
-
 ?
ATP + D-mannose
-
 Escherichia coli ADP + D-mannose 6-phosphate
-
 ?
ATP + D-mannose
-
 Escherichia coli ADP + mannose 6-phosphate
-
 ?
ATP + mannoheptulose no activity Escherichia coli ADP + mannoheptulose 6-phosphate
-
 ?
CTP + D-mannose 15% of the activity with ATP Escherichia coli CDP + D-mannose 6-phosphate
-
 ?
dATP + D-mannose
-
 Escherichia coli dADP + D-mannose 6-phosphate
-
 ?
GTP + D-mannose 60% of the activity with ATP Escherichia coli GDP + D-mannose 6-phosphate
-
 ?
ITP + D-mannose as active as ATP Escherichia coli IDP + D-mannose 6-phosphate
-
 ?
TTP + D-mannose 30% of the activity with ATP Escherichia coli TDP + D-mannose 6-phosphate
-
 ?
UTP + D-mannose 60% of the activity with ATP Escherichia coli UDP + D-mannose 6-phosphate
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
57
-
 5 min, crude extract stable Escherichia coli
61
-
 5 min, crude extract, 10% loss of activity Escherichia coli
64
-
 5 min, crude extract, about 80% loss of activity Escherichia coli
66
-
 5 min, crude extract, complete loss of activity Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
-
 Escherichia coli

pH Stability

pH Stability pH Stability Maximum Comment Organism
4
-
 5 min, 50% loss of activity, 15 min, complete loss of activity Escherichia coli
5 10 25°C, 60 min, stable Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.1
-
 MgADP-
-
 Escherichia coli
0.1
-
 D-mannosamine pH 7.5, 25°C Escherichia coli
2
-
 L-sorbose pH 7.5, 25°C Escherichia coli
5
-
 lyxose pH 7.5, 25°C Escherichia coli