Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-mannose 6-phosphate | competitive versus mannose and noncompetitive versus MgATP2- | Streptomyces sp. | |
MgADP- | - |
Streptomyces sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.33 | - |
D-mannose | pH 8.4, 37°C | Streptomyces sp. | |
1.1 | - |
ATP | pH 8.4, 37°C | Streptomyces sp. | |
1.1 | - |
ATP | ATP in form of MgATP2- | Streptomyces sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Streptomyces sp. | |
Mg2+ | optimal Mg2+/ATP ratio is 1 | Streptomyces sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
gel filtration | Streptomyces sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-mannose | Streptomyces sp. | inducible enzyme | ADP + D-mannose 6-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces sp. | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + D-mannose = ADP + D-mannose 6-phosphate | sequential bi-bi mechanism with two dead-end ternary complexes | Streptomyces sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | grown on D-mannose or beta-D-mannan | Streptomyces sp. | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose | no activity | Streptomyces sp. | ADP + D-fructose 6-phosphate | - |
? | |
ATP + D-mannose | inducible enzyme | Streptomyces sp. | ADP + D-mannose 6-phosphate | - |
? | |
ATP + D-mannose | highly specific for D-mannose | Streptomyces sp. | ADP + mannose 6-phosphate | - |
? | |
CTP + D-mannose | no activity | Streptomyces sp. | CDP + D-mannose 6-phosphate | - |
? | |
GTP + D-mannose | 10% of the activity with ATP | Streptomyces sp. | GDP + D-mannose 6-phosphate | - |
? | |
ITP + D-mannose | 15% of the activity with ATP | Streptomyces sp. | IDP + D-mannose 6-phosphate | - |
? | |
UTP + D-mannose | no activity | Streptomyces sp. | UDP + D-mannose 6-phosphate | - |
? |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
MgADP- | pH 8.4, 37°C, competitive versus MgAtP2- | Streptomyces sp. | |
1.1 | - |
MgADP- | pH 8.4, 37°C, noncompetitive versus mannose | Streptomyces sp. | |
12.5 | - |
mannose 6-phosphate | pH 8.4, 37°C, noncompetitive versus MgATP2- | Streptomyces sp. | |
22.5 | - |
mannose 6-phosphate | pH 8.4, 37°C, competitive versus mannose | Streptomyces sp. |