Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.1.68 extracted from

  • Sharma, S.; Bhattacharya, S.; Bhattacharya, A.
    PtdIns(4,5)P2 is generated by a novel phosphatidylinositol 4-phosphate 5-kinase in the protist parasite Entamoeba histolytica (2019), FEBS J., 286, 2216-2234 .
    View publication on PubMed
Search for more literature for 2.7.1.68

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL-21(DE3) cells Entamoeba histolytica

Protein Variants

Protein Variants Comment Organism
D214A the mutant displays a complete loss of activity Entamoeba histolytica
D276A the mutant displays a complete loss of activity Entamoeba histolytica
K147A the mutant displays a complete loss of activity Entamoeba histolytica

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol faint staining in the cytosol Entamoeba histolytica 5829
-
plasma membrane substantial staining at the plasma membrane Entamoeba histolytica 5886
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ the activity of the enzyme is highest in the presence of Mn2+ and Mg2+ Entamoeba histolytica
Mn2+ the activity of the enzyme is highest in the presence of Mn2+ and Mg2+ Entamoeba histolytica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate Entamoeba histolytica
-
 ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
 ?
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate Entamoeba histolytica HM1:IMSS
-
 ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Entamoeba histolytica C4LSF7
-
-
Entamoeba histolytica HM1:IMSS C4LSF7
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography or glutathione-Sepharose column chromatography Entamoeba histolytica

Source Tissue

Source Tissue Comment Organism Textmining
trophozoite
-
 Entamoeba histolytica
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate weak activity Entamoeba histolytica ADP + 1-phosphatidyl-1D-myo-inositol 3,4,5-triphosphate
-
 ?
ATP + 1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate weak activity Entamoeba histolytica HM1:IMSS ADP + 1-phosphatidyl-1D-myo-inositol 3,4,5-triphosphate
-
 ?
ATP + 1-phosphatidyl-1D-myo-inositol 3-phosphate
-
 Entamoeba histolytica ADP + 1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate
-
 ?
ATP + 1-phosphatidyl-1D-myo-inositol 3-phosphate
-
 Entamoeba histolytica HM1:IMSS ADP + 1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate
-
 ?
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
 Entamoeba histolytica ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
 ?
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate preferred substrate Entamoeba histolytica ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
 ?
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
 Entamoeba histolytica HM1:IMSS ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
 ?
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate preferred substrate Entamoeba histolytica HM1:IMSS ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
 ?
ATP + 1-phosphatidyl-1D-myo-inositol 5-phosphate
-
 Entamoeba histolytica ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
 ?
ATP + 1-phosphatidyl-1D-myo-inositol 5-phosphate
-
 Entamoeba histolytica HM1:IMSS ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 100000, GST-tagged enzyme, calculated from amino acid sequence Entamoeba histolytica
? x * 120000, GST-tagged enzyme, SDS-PAGE Entamoeba histolytica

Synonyms

Synonyms Comment Organism
phosphatidylinositol 4-phosphate 5-kinase
-
 Entamoeba histolytica
PIP5Kalpha isoform Entamoeba histolytica
PIP5Kbeta isoform Entamoeba histolytica
PIP5Kgamma isoform Entamoeba histolytica
PIPKI
-
 Entamoeba histolytica
PtdIns(4)P kinase
-
 Entamoeba histolytica