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Literature summary for 2.7.1.68 extracted from
- Phosphatidylinositol-4-phosphate 5-kinase isoforms exhibit acyl chain selectivity for both substrate and lipid activator (2012), J. Biol. Chem., 287, 35953-35963.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 1,2-diarachidonoyl phosphatidic acid | - |
Mus musculus |  |
| 1,2-dilinoleoyl phosphatidic acid | - |
Mus musculus | |
| 1,2-dioleoyl phosphatidic acid | - |
Mus musculus | |
| 1-arachidoyl-2-arachidonoyl phosphatidic acid | - |
Mus musculus | |
| 1-stearoyl-2-arachidonoyl phosphatidic acid | - |
Mus musculus | |
| 1-stearoyl-2-oleoyl phosphatidic acid | - |
Mus musculus | |
| additional information | PIP5K activation depends on the acyl chain composition of both substrate and activator | Mus musculus | |
| phosphatidic acid | - |
Mus musculus | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of HA-tagged PIP5K isozymes in COS-7 cells, expression of isozyme PIP5Kalpha mutant D322A | Mus musculus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D322A | isozyme PIP5Kalpha mutant that shows reduced activity compared to the wild-type enzyme | Mus musculus |
| L202I | the mutations of PIP5Kalpha decreases the substrate affinity and the enzyme efficiency for 1-stearoyl-2-arachidonoyl phosphatidylinositol-4-phosphate compared to the wild-type enzyme | Mus musculus |
| L210I | the mutations of PIP5Kalpha decreases the substrate affinity and the enzyme efficiency for 1-stearoyl-2-arachidonoyl phosphatidylinositol-4-phosphate compared to the wild-type enzyme | Mus musculus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.6 | - |
1-stearoyl-2-oleoyl phosphatidyl-1D-myo-inositol 4-phosphate | isozyme PIP5Kgamma, pH 7.5, temperature not specified in the publication | Mus musculus | |
| 2.8 | - |
1-stearoyl-2-oleoyl phosphatidyl-1D-myo-inositol 4-phosphate | isozyme PIP5Kalpha, pH 7.5, temperature not specified in the publication | Mus musculus | |
| 3.7 | - |
1-stearoyl-2-oleoyl phosphatidyl-1D-myo-inositol 4-phosphate | isozyme PIP5Kbeta, pH 7.5, temperature not specified in the publication | Mus musculus | |
| 4.9 | - |
1-stearoyl-2-arachidonoyl phosphatidyl-1D-myo-inositol 4-phosphate | isozyme PIP5Kbeta, pH 7.5, temperature not specified in the publication | Mus musculus | |
| 15 | - |
1-stearoyl-2-arachidonoyl phosphatidyl-1D-myo-inositol 4-phosphate | isozyme PIP5Kgamma, pH 7.5, temperature not specified in the publication | Mus musculus | |
| 16 | - |
1-stearoyl-2-arachidonoyl phosphatidyl-1D-myo-inositol 4-phosphate | isozyme PIP5Kalpha, pH 7.5, temperature not specified in the publication | Mus musculus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | isozyme PIP5Kalpha | Mus musculus | 16020 | - |
| nucleus | isozyme PIP5Kalpha | Mus musculus | 5634 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Mus musculus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate | Mus musculus | - |
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| muscle cell | - |
Mus musculus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 1,2-dipalmitoyl phosphatidyl-1D-myo-inositol 4,5-bisphosphate | - |
Mus musculus | ADP + 1,2-dipalmitoyl phosphatidyl-1D-myo-inositol 4,5-bisphosphate | - |
? | |
| ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate | - |
Mus musculus | ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | - |
? | |
| ATP + 1-stearoyl-2-arachidonoyl phosphatidyl-1D-myo-inositol 4-phosphate | - |
Mus musculus | ADP + 1-stearoyl-2-arachidonoyl phosphatidyl-1D-myo-inositol 4,5-bisphosphate | - |
? | |
| ATP + 1-stearoyl-2-oleoyl phosphatidyl-1D-myo-inositol 4-phosphate | - |
Mus musculus | ADP + 1-stearoyl-2-oleoyl phosphatidyl-1D-myo-inositol 4,5-bisphosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| phosphatidylinositol-4-phosphate 5-kinase | - |
Mus musculus |
| PIP5K | - |
Mus musculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Mus musculus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Mus musculus | |
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