Activating Compound | Comment | Organism | Structure |
---|---|---|---|
spermine | 0.5 mM, 300% increase in activity | Schizosaccharomyces pombe |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | 0.1 mM, 55% inhibition | Schizosaccharomyces pombe | |
phosphatidic acid | 0.1 mM, 125% stimulation | Schizosaccharomyces pombe | |
phosphatidylinositol | 0.1 mM, 125% stimulation | Schizosaccharomyces pombe | |
phosphatidylserine | 0.1 mM, 125% stimulation | Schizosaccharomyces pombe | |
spermine | 0.03 mg/ml, 50% inhibition | Schizosaccharomyces pombe |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km for 1-phosphatidyl-1D-myo-inositol 4-phosphate is 1.0 mol% | Schizosaccharomyces pombe | |
0.008 | - |
ATP | pH 7.5 | Schizosaccharomyces pombe |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
plasma membrane | - |
Schizosaccharomyces pombe | 5886 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
63000 | - |
x * 63000, SDS-PAGE | Schizosaccharomyces pombe |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate | Schizosaccharomyces pombe | the enzyme catalyzes the last step in the synthesis of phosphatidylinositol 4,5-bisphosphate, which is a precursor of diacylglycerol and inositol 1,4,5-triphosphate and is also involved in regulation of actin cytoskeleton remodeling and membrane traffic. The enzyme is regulated by casein kinase Cki1 | ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Schizosaccharomyces pombe | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | phosphorylation by Cki1 in vitro decreases the activity of the enzyme | Schizosaccharomyces pombe |
Purification (Comment) | Organism |
---|---|
- |
Schizosaccharomyces pombe |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.704 | - |
- |
Schizosaccharomyces pombe |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate | - |
Schizosaccharomyces pombe | ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | - |
? | |
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate | the enzyme catalyzes the last step in the synthesis of phosphatidylinositol 4,5-bisphosphate, which is a precursor of diacylglycerol and inositol 1,4,5-triphosphate and is also involved in regulation of actin cytoskeleton remodeling and membrane traffic. The enzyme is regulated by casein kinase Cki1 | Schizosaccharomyces pombe | ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 63000, SDS-PAGE | Schizosaccharomyces pombe |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Schizosaccharomyces pombe |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8.5 | pH 5.5: about 35% of maximal activity, pH 8.5: about 60% of maximal activity | Schizosaccharomyces pombe |