Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.1.68 extracted from

  • Cochet, C.; Chambaz, E.M.
    Catalytic properties of a purified phosphatidylinositol-4-phosphate kinase from rat brain (1986), Biochem. J., 237, 25-31.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.68

Activating Compound

Activating Compound Comment Organism Structure
spermine strong activation in presence of micromolar concentrations, at 2 mM Mg2+ optimal spermine concentration is 0.1-0.2 mM spermine Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
heparin IC50: 0.002 mg/ml, competitive towards 1-phosphatidyl-1D-myo-inositol 4-phosphate Rattus norvegicus
quercetin IC50: 0.0002 mM, competitive towards ATP or GTP Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km-value for 1-phosphatidyl-1D-myo-inositol 4-phosphate is 0.0033 mg/ml Rattus norvegicus
0.025
-
 ATP pH 7.5, 22°C Rattus norvegicus
0.133
-
 GTP pH 7.5, 22°C Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required, optimal concentration is 20-30 mM Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1
-
-
 Rattus norvegicus

Storage Stability

Storage Stability Organism
-80°C, stable for at least 3 months Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
 Rattus norvegicus ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
 ?
GTP + 1-phosphatidyl-1D-myo-inositol 4-phosphate 50% of the activity with ATP Rattus norvegicus GDP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
 ?