Activating Compound | Comment | Organism | Structure |
---|---|---|---|
heparin | type I enzyme is stimulated at low concentrations | Homo sapiens | |
spermine | 2 mM, 4fold increase in activity of type I enzyme, no effect on type II enzyme | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
heparin | type II kinase | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0012 | - |
1-phosphatidyl-1D-myo-inositol 4-phosphate | in 1-phosphatidyl-1D-myo-inositol 4-phosphate in membranes, type I enzyme | Homo sapiens | |
0.0014 | - |
1-phosphatidyl-1D-myo-inositol 4-phosphate | in 1-phosphatidyl-1D-myo-inositol 4-phosphate liposomes, type I enzyme | Homo sapiens | |
0.005 | - |
ATP | type II enzyme | Homo sapiens | |
0.006 | - |
1-phosphatidyl-1D-myo-inositol 4-phosphate | in 1-phosphatidyl-1D-myo-inositol 4-phosphate micelles, type I enzyme | Homo sapiens | |
0.025 | - |
ATP | type I enzyme | Homo sapiens | |
0.04 | - |
1-phosphatidyl-1D-myo-inositol 4-phosphate | in 1-phosphatidyl-1D-myo-inositol 4-phosphate liposomes, type II enzyme | Homo sapiens | |
0.06 | - |
1-phosphatidyl-1D-myo-inositol 4-phosphate | in 1-phosphatidyl-1D-myo-inositol 4-phosphate micelles, type II enzyme | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | type I kinase is membrane bound and type II kinase is both membrane bound and cytosolic | Homo sapiens | 5829 | - |
membrane | type I kinase is membrane bound and type II kinase is both membrane bound and cytosolic | Homo sapiens | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
x * 53000, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
cytosolic enzyme type II enzyme and membrane-bound type I and type II enzyme | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
erythrocyte | two form of PIP kinase: type I kinase is membrane bound and type II kinase is both membrane bound and cytosolic | Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.015 | - |
membrane bound type II enzyme | Homo sapiens |
0.023 | - |
cytosolic enzyme | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate | - |
Homo sapiens | ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | - |
? | |
GTP + 1-phosphatidyl-1D-myo-inositol 4-phosphate | 0.05 mM GTP is 2fold more active than ATP with type II enzyme. 0.05 mM GTP is 5fold more active than ATP with the type I enzyme | Homo sapiens | GDP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 53000, SDS-PAGE | Homo sapiens |