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Literature summary for 2.7.1.67 extracted from

  • Dumaresq-Doiron, K.; Savard, M.F.; Akam, S.; Costantino, S.; Lefrancois, S.
    The phosphatidylinositol 4-kinase PI4KIIIalpha is required for the recruitment of GBF1 to Golgi membranes (2010), J. Cell Sci., 123, 2273-2280.
    View publication on PubMed
Search for more literature for 2.7.1.67

Activating Compound

Activating Compound Comment Organism Structure
RAB1 activates PI4KIIIalpha and stimulates the production of phosphatidylinositol 4-phosphate at the Golgi Saccharomyces cerevisiae

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression and co-localization of fluorescent-labeled GBF1 with the fluorescent-labeled PI4KIIIalpha in COS-7 cells Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
additional information depletion of isozymes PI4KIIIalpha and PI4KIIIbeta using siRNA, depletion of PI4KIIIalpha prevents the recruitment of GBF1 to Golgi membranes,phenotypes, overview Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
LY294002
-
 Saccharomyces cerevisiae
PAO inhibitor of type III PI4Ks, with greater specificity for PI4KIIIalpha than for PI4KIIIbeta Saccharomyces cerevisiae
Wortmannin inhibitor of type III PI4Ks Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum PI4KIIIalpha Saccharomyces cerevisiae 5783
-
Golgi apparatus PI4KIIIalpha Saccharomyces cerevisiae 5794
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 1-phosphatidyl-1D-myo-inositol Saccharomyces cerevisiae
-
 ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1-phosphatidyl-1D-myo-inositol
-
 Saccharomyces cerevisiae ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
 ?

Synonyms

Synonyms Comment Organism
phosphatidylinositol 4-kinase
-
 Saccharomyces cerevisiae
PI4KIIIalpha
-
 Saccharomyces cerevisiae
type III PI4K
-
 Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Saccharomyces cerevisiae

General Information

General Information Comment Organism
malfunction Inhibitors of phosphatidylinositol 4-phosphate synthesis or depletion of PI4KIIIalpha, a phosphatidylinositol 4-kinase localized to the endoplasmic reticulum and Golgi, prevents the recruitment of GBF1 to Golgi membranes Saccharomyces cerevisiae
physiological function phosphatidylinositol 4-phosphate, synthesized by phosphatidylinositol 4-kinase isozyme PI4KIIIalpha, is required for recruitment of the guanine nucleotide exchange factor GBF1 to Golgi membranes, where it is bound and activates Arf1. Activated Arf1 is required for formation of trafficking vesicles in the Golgi apparatus, overview. Rab1 contributes to the specificity and timing of GBF1 recruitment by activating PI4KIIIalpha Saccharomyces cerevisiae