Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 10 mM, 50% inhibition | Saccharomyces cerevisiae | |
Hg2+ | - |
Saccharomyces cerevisiae | |
NEM | - |
Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
phosphatidylinositol | pH 8.0, 30°C | Saccharomyces cerevisiae | |
0.3 | - |
ATP | pH 8.0, 30°C | Saccharomyces cerevisiae | |
0.3 | - |
ATP | ATP in form of MgATP2- | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | dependent on | Saccharomyces cerevisiae | |
Mn2+ | can not substitute for Mg2+ | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
x * 35000, SDS-PAGE | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate | sequential reaction mechanism | Saccharomyces cerevisiae |
Storage Stability | Organism |
---|---|
-80°C, purified enzyme is completely stable for at least 2 months | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1-phosphatidyl-1D-myo-inositol | reverse reaction is facored in vitro | Saccharomyces cerevisiae | ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 35000, SDS-PAGE | Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Saccharomyces cerevisiae |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 50 | 20°C: about 20% of maximal activity, 50°C: about 15% of maximal activity | Saccharomyces cerevisiae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
unstable above | Saccharomyces cerevisiae |
50 | - |
20 min, complete inactivation | Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.77 | - |
ATP | pH 8.0, 30°C | Saccharomyces cerevisiae | |
2.77 | - |
1-phosphatidyl-1D-myo-inositol | pH 8.0, 30°C | Saccharomyces cerevisiae |