Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.1.67 extracted from

  • Belunis, C.J.; Bae-Lee, M.; Kelley, M.J.; Carman, G.M.
    Purification and characterization of phosphatidylinositol kinase from Saccharomyces cerevisiae (1988), J. Biol. Chem., 263, 18897-18903.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 10 mM, 50% inhibition Saccharomyces cerevisiae
Hg2+
-
Saccharomyces cerevisiae
NEM
-
Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
phosphatidylinositol pH 8.0, 30°C Saccharomyces cerevisiae
0.3
-
ATP pH 8.0, 30°C Saccharomyces cerevisiae
0.3
-
ATP ATP in form of MgATP2- Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ dependent on Saccharomyces cerevisiae
Mn2+ can not substitute for Mg2+ Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
x * 35000, SDS-PAGE Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharomyces cerevisiae

Reaction

Reaction Comment Organism Reaction ID
ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate sequential reaction mechanism Saccharomyces cerevisiae

Storage Stability

Storage Stability Organism
-80°C, purified enzyme is completely stable for at least 2 months Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1-phosphatidyl-1D-myo-inositol reverse reaction is facored in vitro Saccharomyces cerevisiae ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
r

Subunits

Subunits Comment Organism
? x * 35000, SDS-PAGE Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Saccharomyces cerevisiae

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 50 20°C: about 20% of maximal activity, 50°C: about 15% of maximal activity Saccharomyces cerevisiae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
unstable above Saccharomyces cerevisiae
50
-
20 min, complete inactivation Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.77
-
ATP pH 8.0, 30°C Saccharomyces cerevisiae
2.77
-
1-phosphatidyl-1D-myo-inositol pH 8.0, 30°C Saccharomyces cerevisiae