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Literature summary for 2.7.1.67 extracted from
- Partial purification and characterization of phosphatidylinositol kinases from human platelets (1990), Biochim. Biophys. Acta, 1046, 120-126.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Triton X-100 | inhibits activity of mPIK-I but rather weakly enhances mPIK-III activity | Homo sapiens |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | inhibition of mPIK-I and mPIK-III | Homo sapiens | |
| arachidonic acid | 0.5 mg/ml, 91.3% inhibition of mPIK-III | Homo sapiens | |
| phosphatidylserine | 0.5 mg/ml, 64.3% inhibition of mPIK-III | Homo sapiens | |
| sodium cholate | inhibits activity of both mPIK-I and mPIK-III | Homo sapiens | |
| Triton X-100 | inhibits activity of mPIK-I but rather weakly enhances mPIK-III activity | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.075 | - |
ATP | pH 7.4, 30°C, enzyme form mPIK-III | Homo sapiens | |
| 0.152 | - |
ATP | pH 7.4, 30°C, enzyme form cPIK-I and cPIK-II | Homo sapiens | |
| 0.217 | - |
ATP | pH 7.4, 30°C, enzyme form mPIK-I | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | enzyme form cPIK-I and cPIK-II | Homo sapiens | 5829 | - |
| membrane | enzyme form mPIK-I and mPIK-II | Homo sapiens | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required, optimal concentrations for mPIK-I and mPIK-III at 5-10 mM, optimal concentration for cPIK-I and cPIK-II is 20-40 mM | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 76000 | - |
mPIK-I, gel filtration | Homo sapiens |
| 80000 | - |
mPIK-III, gel filtration | Homo sapiens |
| 550000 | - |
cPIK-I and cPIK-II, gel filtration | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| mPIK-I and mPIK-III from membrane fraction and cPIK-I and cPIK-II from cytoslic fraction | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| platelet | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 1-phosphatidyl-1D-myo-inositol | - |
Homo sapiens | ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
cPIK-I and cPIK-II | Homo sapiens |
| 8.5 | 9 | mPIK-I and mPIK-III | Homo sapiens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9 | pH 5.5: 60% of maximal activity for mPIK-I and 45% of maximal activity of mPIK-III, pH 8.5-9.0: pH-optimum for enzyme form mPIK-I and mPIK-III | Homo sapiens |
| 6 | 9 | activity of cPIK-I and cPIK-III is almost undetectable at pH 6.0, pH 9.0: about 80% of maximal activity | Homo sapiens |
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