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Literature summary for 2.7.1.67 extracted from

  • Porter, F.D.; Li, Y.S.; Deuel, T.F.
    Purification and characterization of a phosphatidylinositol 4-kinase from bovine uteri (1988), J. Biol. Chem., 263, 8989-8995.
    View publication on PubMed
Search for more literature for 2.7.1.67

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 0.3 mM, 50% inhibition. Increasing Mg2+ concentrations antagonize this inhibition Bos taurus
phosphatidylinositol 4,5-bisphosphate 0.5 mg/ml, 59% inhibition Bos taurus
phosphatidylinositol 4-phosphate 0.5 mg/ml, 42% inhibition Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.018
-
 ATP pH 7.0, 30°C Bos taurus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activates, maximal activity at 2-10 mM Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 1 * 55000, SDS-PAGE Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
uterus
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7
-
-
 Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + 1-phosphatidyl-1D-myo-inositol 12% of the activity with ATP Bos taurus AMP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
 ?
ATP + 1-phosphatidyl-1D-myo-inositol
-
 Bos taurus ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
 ?
CTP + 1-phosphatidyl-1D-myo-inositol 10% of the activity with ATP Bos taurus CDP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
 ?
dATP + 1-phosphatidyl-1D-myo-inositol 104% of the activity with ATP Bos taurus dADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
 ?
GTP + 1-phosphatidyl-1D-myo-inositol 5% of the activity with ATP Bos taurus GDP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
 ?
ITP + 1-phosphatidyl-1D-myo-inositol 11% of the activity with ATP Bos taurus IDP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
 ?
TTP + 1-phosphatidyl-1D-myo-inositol 3% of the activity with ATP Bos taurus TDP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
 ?
UTP + 1-phosphatidyl-1D-myo-inositol 18% of the activity with ATP Bos taurus UDP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 55000, SDS-PAGE Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7
-
 Bos taurus

pH Range

pH Minimum pH Maximum Comment Organism
5 8 pH 5.0: about 40% of maximal activity, pH 8.0: about 50% of maximal activity Bos taurus