BRENDA - Enzyme Database show
show all sequences of 2.7.1.64

An uncharacterized member of the ribokinase family in Thermococcus kodakarensis exhibits myo-inositol kinase activity

Sato, T.; Fujihashi, M.; Miyamoto, Y.; Kuwata, K.; Kusaka, E.; Fujita, H.; Miki, K.; Atomi, H.; J. Biol. Chem. 288, 20856-20867 (2013)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL and Rosetta2(DE3)pLysS cells
Thermococcus kodakarensis
Crystallization (Commentary)
Crystallization
Organism
sitting drop vapor diffusion method, usingv 18-23% (w/v) polyethylene glycol 3350 and 0.2 M NH4I
Thermococcus kodakarensis
Inhibitors
Inhibitors
Commentary
Organism
Structure
Ca2+
49% residual activity at 3 mM
Thermococcus kodakarensis
EDTA
-
Thermococcus kodakarensis
Mn2+
21% residual activity at 3 mM
Thermococcus kodakarensis
Ni2+
53% residual activity at 3 mM
Thermococcus kodakarensis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.36
-
myo-inositol
at pH 7.5 and 85°C
Thermococcus kodakarensis
53
-
D-tagatose
at pH 7.5 and 85°C
Thermococcus kodakarensis
171
-
D-Lyxose
at pH 7.5 and 85°C
Thermococcus kodakarensis
418
-
D-xylose
at pH 7.5 and 85°C
Thermococcus kodakarensis
438
-
D-talose
at pH 7.5 and 85°C
Thermococcus kodakarensis
524
-
D-xylulose
at pH 7.5 and 85°C
Thermococcus kodakarensis
863
-
D-allose
at pH 7.5 and 85°C
Thermococcus kodakarensis
951
-
D-ribose
at pH 7.5 and 85°C
Thermococcus kodakarensis
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Co2+
133% activity at 3 mM
Thermococcus kodakarensis
Zn2+
125% activity at 3 mM
Thermococcus kodakarensis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
30300
-
-
Thermococcus kodakarensis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + myo-inositol
Thermococcus kodakarensis
-
ADP + 1D-myo-inositol 3-phosphate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Thermococcus kodakarensis
Q5JDA3
-
-
Purification (Commentary)
Commentary
Organism
Resource Q column chromatography, Resource PHE hydrophobic column chromatography, and Superdex 200 gel filtration
Thermococcus kodakarensis
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.063
-
with meso-erythritol as substrate, at pH 7.5 and 85°C
Thermococcus kodakarensis
0.098
-
with L-arabitol as substrate, at pH 7.5 and 85°C
Thermococcus kodakarensis
0.17
-
with cis-inositol as substrate, at pH 7.5 and 85°C
Thermococcus kodakarensis
0.24
-
with L-chiro-inositol as substrate, at pH 7.5 and 85°C
Thermococcus kodakarensis
0.39
-
with adonitol as substrate, at pH 7.5 and 85°C
Thermococcus kodakarensis
0.5
-
with D-arabitol as substrate, at pH 7.5 and 85°C
Thermococcus kodakarensis
1.06
-
with myo-inositol 1-phosphate as substrate, at pH 7.5 and 85°C
Thermococcus kodakarensis
1.91
-
with allo-inositol as substrate, at pH 7.5 and 85°C
Thermococcus kodakarensis
17.64
-
with scyllo-inositol as substrate, at pH 7.5 and 85°C
Thermococcus kodakarensis
34.92
-
with D-chiro-inositol as substrate, at pH 7.5 and 85°C
Thermococcus kodakarensis
44.27
-
with myo-inositol as substrate, at pH 7.5 and 85°C
Thermococcus kodakarensis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + D-allose
-
738585
Thermococcus kodakarensis
?
-
-
-
?
ATP + D-lyxose
-
738585
Thermococcus kodakarensis
?
-
-
-
?
ATP + D-ribose
-
738585
Thermococcus kodakarensis
?
-
-
-
?
ATP + D-tagatose
-
738585
Thermococcus kodakarensis
?
-
-
-
?
ATP + D-talose
-
738585
Thermococcus kodakarensis
?
-
-
-
?
ATP + D-xylose
-
738585
Thermococcus kodakarensis
?
-
-
-
?
ATP + D-xylulose
-
738585
Thermococcus kodakarensis
?
-
-
-
?
ATP + myo-inositol
-
738585
Thermococcus kodakarensis
ADP + 1D-myo-inositol 3-phosphate
-
-
-
?
additional information
no activity with glycerol, DL-threitol, L-iditol, dulcitol, volemitol, D-lactitol monohydrate, maltitol, D-glucosamine, N-acetyl-D-glucosamine, maltose and lactose, D-erythrose, D-threose, D-erythrulose, L-arabinose, L-fructose, L-glucose, D-ribulose, D-galactose, fructose 1-phosphate, fructose 6-phosphate, glucose 1-phosphate, glucose 6-phosphate, ribose 1-phosphate, ribose 5-phosphate, tagatose 6-phosphate, adenosine, uridine, guanosine, cytidine, thymidine, and inosine
738585
Thermococcus kodakarensis
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 30300, calculated from amino acid sequence
Thermococcus kodakarensis
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
85
-
-
Thermococcus kodakarensis
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
65
95
the enzyme is extremely thermostable with half-lives of 3 h at 95°C, 19 h at 85°C, 40 h at 75°C, and 88 h at 65°C
Thermococcus kodakarensis
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.5
-
D-tagatose
at pH 7.5 and 85°C
Thermococcus kodakarensis
3.9
-
D-Lyxose
at pH 7.5 and 85°C
Thermococcus kodakarensis
5.1
-
D-ribose
at pH 7.5 and 85°C
Thermococcus kodakarensis
5.6
-
D-xylose
at pH 7.5 and 85°C
Thermococcus kodakarensis
11.6
-
D-xylulose
at pH 7.5 and 85°C
Thermococcus kodakarensis
13.1
-
D-talose
at pH 7.5 and 85°C
Thermococcus kodakarensis
18.3
-
D-allose
at pH 7.5 and 85°C
Thermococcus kodakarensis
23.2
-
myo-inositol
at pH 7.5 and 85°C
Thermococcus kodakarensis
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL and Rosetta2(DE3)pLysS cells
Thermococcus kodakarensis
Crystallization (Commentary) (protein specific)
Crystallization
Organism
sitting drop vapor diffusion method, usingv 18-23% (w/v) polyethylene glycol 3350 and 0.2 M NH4I
Thermococcus kodakarensis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ca2+
49% residual activity at 3 mM
Thermococcus kodakarensis
EDTA
-
Thermococcus kodakarensis
Mn2+
21% residual activity at 3 mM
Thermococcus kodakarensis
Ni2+
53% residual activity at 3 mM
Thermococcus kodakarensis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.36
-
myo-inositol
at pH 7.5 and 85°C
Thermococcus kodakarensis
53
-
D-tagatose
at pH 7.5 and 85°C
Thermococcus kodakarensis
171
-
D-Lyxose
at pH 7.5 and 85°C
Thermococcus kodakarensis
418
-
D-xylose
at pH 7.5 and 85°C
Thermococcus kodakarensis
438
-
D-talose
at pH 7.5 and 85°C
Thermococcus kodakarensis
524
-
D-xylulose
at pH 7.5 and 85°C
Thermococcus kodakarensis
863
-
D-allose
at pH 7.5 and 85°C
Thermococcus kodakarensis
951
-
D-ribose
at pH 7.5 and 85°C
Thermococcus kodakarensis
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Co2+
133% activity at 3 mM
Thermococcus kodakarensis
Zn2+
125% activity at 3 mM
Thermococcus kodakarensis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
30300
-
-
Thermococcus kodakarensis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + myo-inositol
Thermococcus kodakarensis
-
ADP + 1D-myo-inositol 3-phosphate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
Resource Q column chromatography, Resource PHE hydrophobic column chromatography, and Superdex 200 gel filtration
Thermococcus kodakarensis
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.063
-
with meso-erythritol as substrate, at pH 7.5 and 85°C
Thermococcus kodakarensis
0.098
-
with L-arabitol as substrate, at pH 7.5 and 85°C
Thermococcus kodakarensis
0.17
-
with cis-inositol as substrate, at pH 7.5 and 85°C
Thermococcus kodakarensis
0.24
-
with L-chiro-inositol as substrate, at pH 7.5 and 85°C
Thermococcus kodakarensis
0.39
-
with adonitol as substrate, at pH 7.5 and 85°C
Thermococcus kodakarensis
0.5
-
with D-arabitol as substrate, at pH 7.5 and 85°C
Thermococcus kodakarensis
1.06
-
with myo-inositol 1-phosphate as substrate, at pH 7.5 and 85°C
Thermococcus kodakarensis
1.91
-
with allo-inositol as substrate, at pH 7.5 and 85°C
Thermococcus kodakarensis
17.64
-
with scyllo-inositol as substrate, at pH 7.5 and 85°C
Thermococcus kodakarensis
34.92
-
with D-chiro-inositol as substrate, at pH 7.5 and 85°C
Thermococcus kodakarensis
44.27
-
with myo-inositol as substrate, at pH 7.5 and 85°C
Thermococcus kodakarensis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + D-allose
-
738585
Thermococcus kodakarensis
?
-
-
-
?
ATP + D-lyxose
-
738585
Thermococcus kodakarensis
?
-
-
-
?
ATP + D-ribose
-
738585
Thermococcus kodakarensis
?
-
-
-
?
ATP + D-tagatose
-
738585
Thermococcus kodakarensis
?
-
-
-
?
ATP + D-talose
-
738585
Thermococcus kodakarensis
?
-
-
-
?
ATP + D-xylose
-
738585
Thermococcus kodakarensis
?
-
-
-
?
ATP + D-xylulose
-
738585
Thermococcus kodakarensis
?
-
-
-
?
ATP + myo-inositol
-
738585
Thermococcus kodakarensis
ADP + 1D-myo-inositol 3-phosphate
-
-
-
?
additional information
no activity with glycerol, DL-threitol, L-iditol, dulcitol, volemitol, D-lactitol monohydrate, maltitol, D-glucosamine, N-acetyl-D-glucosamine, maltose and lactose, D-erythrose, D-threose, D-erythrulose, L-arabinose, L-fructose, L-glucose, D-ribulose, D-galactose, fructose 1-phosphate, fructose 6-phosphate, glucose 1-phosphate, glucose 6-phosphate, ribose 1-phosphate, ribose 5-phosphate, tagatose 6-phosphate, adenosine, uridine, guanosine, cytidine, thymidine, and inosine
738585
Thermococcus kodakarensis
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 30300, calculated from amino acid sequence
Thermococcus kodakarensis
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
85
-
-
Thermococcus kodakarensis
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
65
95
the enzyme is extremely thermostable with half-lives of 3 h at 95°C, 19 h at 85°C, 40 h at 75°C, and 88 h at 65°C
Thermococcus kodakarensis
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.5
-
D-tagatose
at pH 7.5 and 85°C
Thermococcus kodakarensis
3.9
-
D-Lyxose
at pH 7.5 and 85°C
Thermococcus kodakarensis
5.1
-
D-ribose
at pH 7.5 and 85°C
Thermococcus kodakarensis
5.6
-
D-xylose
at pH 7.5 and 85°C
Thermococcus kodakarensis
11.6
-
D-xylulose
at pH 7.5 and 85°C
Thermococcus kodakarensis
13.1
-
D-talose
at pH 7.5 and 85°C
Thermococcus kodakarensis
18.3
-
D-allose
at pH 7.5 and 85°C
Thermococcus kodakarensis
23.2
-
myo-inositol
at pH 7.5 and 85°C
Thermococcus kodakarensis
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.005
-
D-ribose
at pH 7.5 and 85°C
Thermococcus kodakarensis
0.013
-
D-xylose
at pH 7.5 and 85°C
Thermococcus kodakarensis
0.021
-
D-allose
at pH 7.5 and 85°C
Thermococcus kodakarensis
0.022
-
D-xylulose
at pH 7.5 and 85°C
Thermococcus kodakarensis
0.023
-
D-Lyxose
at pH 7.5 and 85°C
Thermococcus kodakarensis
0.028
-
D-tagatose
at pH 7.5 and 85°C
Thermococcus kodakarensis
0.03
-
D-talose
at pH 7.5 and 85°C
Thermococcus kodakarensis
64.9
-
myo-inositol
at pH 7.5 and 85°C
Thermococcus kodakarensis
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.005
-
D-ribose
at pH 7.5 and 85°C
Thermococcus kodakarensis
0.013
-
D-xylose
at pH 7.5 and 85°C
Thermococcus kodakarensis
0.021
-
D-allose
at pH 7.5 and 85°C
Thermococcus kodakarensis
0.022
-
D-xylulose
at pH 7.5 and 85°C
Thermococcus kodakarensis
0.023
-
D-Lyxose
at pH 7.5 and 85°C
Thermococcus kodakarensis
0.028
-
D-tagatose
at pH 7.5 and 85°C
Thermococcus kodakarensis
0.03
-
D-talose
at pH 7.5 and 85°C
Thermococcus kodakarensis
64.9
-
myo-inositol
at pH 7.5 and 85°C
Thermococcus kodakarensis
Other publictions for EC 2.7.1.64
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737707
Nagata
Crystal structure and product ...
Thermococcus kodakarensis
Biochemistry
54
3494-3503
2015
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738585
Sato
An uncharacterized member of t ...
Thermococcus kodakarensis
J. Biol. Chem.
288
20856-20867
2013
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1
1
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4
8
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1
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5
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11
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11
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676495
Shi
The maize low-phytic acid 3 en ...
Zea mays
Plant J.
42
708-719
2005
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1
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636604
Loewus
Enantiomeric form of myo-inosi ...
Triticum aestivum
Plant Physiol.
70
1661-1663
1982
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1
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4
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7
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2
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1
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7
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2
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636603
Molinari
Studies on the biosynthesis of ...
Lemna gibba
Hoppe-Seyler's Z. Physiol. Chem.
349
1797-1799
1968
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1
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1
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1
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2
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1
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636602
English
Myoinositol kinase: partial pu ...
Spinacia oleracea, Triticum aestivum, Vigna radiata var. radiata
Science
151
198-199
1966
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3
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3
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636605
Dietz
The enzymic phosphorylation of ...
Acer pseudoplatanus, Bos taurus, Escherichia coli, Vigna radiata var. radiata
Biochem. Biophys. Res. Commun.
19
598-603
1965
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4
-
-
-
-
-
3
-
-
16
-
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
16
-
-
-
-
-
4
1
-
-
-
-
-
-
-
-