Literature summary for 2.7.1.63 extracted from

Reshetnikov, A.; Solntseva, N.; Rozova, O.; Mustakhimov, I.; Trotsenko, Y.; Khmelenina, V.
ATP-and polyphosphate-dependent glucokinases from aerobic methanotrophs (2019), Microorganisms, 7, 52 .

Search for more literature for 2.7.1.63

Activating Compound

Activating Compound	Comment	Organism	Structure
Adenosine triphosphate	5 mM, 1.55fold activation	Methylomonas methanica
glyceraldehyde-3-phosphate	slight activation	Methylomonas methanica
phosphoenolpyruvate	5 mM, 1.11fold activation	Methylomonas methanica
ribose-5-phosphate	5 mM, 1.18fold activation	Methylomonas methanica

Cloned(Commentary)

Cloned (Comment)	Organism
heterologous expression in Esherichia coli	Methylomonas methanica

Inhibitors

Inhibitors	Comment	Organism	Structure
ADP	0.5 M, 7% loss of activity	Methylomonas methanica
AMP	0.5 M, 16% loss of activity	Methylomonas methanica

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.021	-	(phosphate)45	28°C, pH 8.5	Methylomonas methanica
0.45	-	D-glucose	28°C, pH 8.5	Methylomonas methanica

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
6000	-	gel filtration, dimerization in presence of polyP	Methylomonas methanica
30000	-	SDS-PAGE	Methylomonas methanica
30000	-	gel filtration in absence of polyP	Methylomonas methanica

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(phosphate)n + D-glucose	Methylomonas methanica	-	(phosphate)n-1 + D-glucose 6-phosphate	-	?
(phosphate)n + D-glucose	Methylomonas methanica 12	-	(phosphate)n-1 + D-glucose 6-phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Methylomonas methanica	A0A3G4RHV5	-	-
Methylomonas methanica 12	A0A3G4RHV5	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Methylomonas methanica

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(phosphate)45 + D-glucose	-	Methylomonas methanica	(phosphate)44 + D-glucose 6-phosphate	-	?
(phosphate)45 + D-glucose	-	Methylomonas methanica 12	(phosphate)44 + D-glucose 6-phosphate	-	?
(phosphate)n + D-glucose	-	Methylomonas methanica	(phosphate)n-1 + D-glucose 6-phosphate	-	?
(phosphate)n + D-glucose	-	Methylomonas methanica 12	(phosphate)n-1 + D-glucose 6-phosphate	-	?

Subunits

Subunits	Comment	Organism
?	x * 30000, SDS-PAGE, monomer oder dimer, dimerization in in presence of polyP	Methylomonas methanica

Synonyms

Synonyms	Comment	Organism
PolyP-dependent glucokinase	-	Methylomonas methanica
polyP-Glk	-	Methylomonas methanica
polyphosphate-dependent glucokinase	-	Methylomonas methanica
PPGK	-	Methylomonas methanica

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
65	-	-	Methylomonas methanica

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
28	-	2 h, 30% loss of activity	Methylomonas methanica
35	-	2 h, 64% loss of activity	Methylomonas methanica
40	-	2 h, 95% loss of activity	Methylomonas methanica
55	-	30 min, complete inactivation	Methylomonas methanica
60	65	5 min, complete inactivation	Methylomonas methanica

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.0066	-	D-glucose	28°C, pH 8.5	Methylomonas methanica
0.0068	-	(phosphate)45	28°C, pH 8.5	Methylomonas methanica

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.5	-	-	Methylomonas methanica

pH Range

pH Minimum	pH Maximum	Comment	Organism
5.5	10.5	active at a pH range of 5.5-10.5	Methylomonas methanica

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.0145	-	D-glucose	28°C, pH 8.5	Methylomonas methanica
0.33	-	(phosphate)45	28°C, pH 8.5	Methylomonas methanica

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Release 2023.1 (January 2023)